ODP417 Kisspeptin directly stimulates estrogen and progesterone secretion from human granulosa cells.

Abstract Kisspeptin is a peptide hormone that has a central role to stimulate gonadotropin-releasing hormone secretion from the hypothalamus. Furthermore, it was shown to have peripheral roles on enhancing CYP19 (aromatase) expression and aromatase secretion from human granulosa cells (1). The objectives of this study were to determine direct effects of kisspeptin treatment on estrogen and progesterone secretion from human granulosa-like tumor cell line (KGN). KGN cells (n=3) were treated with 10 nM follicle stimulating hormone (FSH); 100, 1,000, and 10,000 nM kisspeptin; and FSH together with different doses of kisspeptin for 24 hours. Estrogen and progesterone concentrations in supernatant were measured by enzyme immunoassay kits. The results showed that FSH treatment alone (P<0. 05) and FSH together with 100 (P<0. 01), 1,000 (P<0. 001), and 10,000 (P<0. 001) nM kisspeptin increased estrogen concentrations in supernatants when compared to control. Kisspeptin treatment alone did not increase estrogen concentrations in supernatant when compared to control. Interestingly, kisspeptin treatment at 1,000 (P<0. 01) and 10,000 (P<0. 01) nM together with FSH enhanced estrogen concentrations in supernatant when compared to FSH treatment alone. Furthermore, FSH treatment alone (P<0. 05) and FSH together with kisspeptin at doses of 1,000 (P<0. 001) and 10,000 (P<0. 05) nM increased progesterone concentrations in supernatant when compared to control. All doses of kisspeptin did not increase progesterone concentrations in supernatant when compared to control. Only 1,000 nM kisspeptin together with FSH enhanced progesterone secretion in supernatant when compared to FSH alone (P<0. 01). Taken together, FSH stimulated estrogen and progesterone secretion from human granulosa cells and kisspeptin enhanced these effects in addition to the effects of FSH alone. Further studies are required to determine whether kisspeptin treatment can restore the functions of unresponsive or partially responsive granulosa cells of aging humans to secrete estrogen and progesterone as well as kisspeptin is useful in augmenting infertility treatment. Reference: (1) Qin L, Sitticharoon C, Petyim S, Keadkraichaiwat I, Sririwichitchai R, Maikeaw P, et al. Exp Biol Med (Maywood). 2021;246(8): 996-1010. Presentation: No date and time listed