Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
ATP‐independent DNA topoisomerase II as potential drug target in trypanosomes
View through CrossRef
Two categories of trypanosomal type II topoisomerases have been isolated from trypanosomes: one is unique since it is able to realize DNA topoisomerization reactions in the absence of ATP, in contrast to the other enzyme and mammalian topoisomerase II. The biochemical properties of ATP‐independent topoisomerase II from Trypanosoma cruzi are described in this report. The enzyme can decatenate trypanosome kinetoplast DNA networks, catenate supercoiled DNA molecules, unknot P4 phage DNA, and cleave double‐stranded DNA. The enzyme is inhibited by various classes of drugs and is more sensitive than mammalian topoisomerase II. Therefore, trypanosome ATP‐independent topoisomerase II provides a potential target for chemotherapy.
Title: ATP‐independent DNA topoisomerase II as potential drug target in trypanosomes
Description:
Two categories of trypanosomal type II topoisomerases have been isolated from trypanosomes: one is unique since it is able to realize DNA topoisomerization reactions in the absence of ATP, in contrast to the other enzyme and mammalian topoisomerase II.
The biochemical properties of ATP‐independent topoisomerase II from Trypanosoma cruzi are described in this report.
The enzyme can decatenate trypanosome kinetoplast DNA networks, catenate supercoiled DNA molecules, unknot P4 phage DNA, and cleave double‐stranded DNA.
The enzyme is inhibited by various classes of drugs and is more sensitive than mammalian topoisomerase II.
Therefore, trypanosome ATP‐independent topoisomerase II provides a potential target for chemotherapy.
Related Results
Topoisomerase Assays
Topoisomerase Assays
AbstractTopoisomerases are enzymes that play essential roles in DNA replication, transcription, chromosome segregation, and recombination. All cells have two major forms of DNA top...
Genome wide hypomethylation and youth-associated DNA gap reduction promoting DNA damage and senescence-associated pathogenesis
Genome wide hypomethylation and youth-associated DNA gap reduction promoting DNA damage and senescence-associated pathogenesis
Abstract
Background: Age-associated epigenetic alteration is the underlying cause of DNA damage in aging cells. Two types of youth-associated DNA-protection epigenetic mark...
Selection of Injectable Drug Product Composition using Machine Learning Models (Preprint)
Selection of Injectable Drug Product Composition using Machine Learning Models (Preprint)
BACKGROUND
As of July 2020, a Web of Science search of “machine learning (ML)” nested within the search of “pharmacokinetics or pharmacodynamics” yielded over 100...
Echinococcus granulosus in Environmental Samples: A Cross-Sectional Molecular Study
Echinococcus granulosus in Environmental Samples: A Cross-Sectional Molecular Study
Abstract
Introduction
Echinococcosis, caused by tapeworms of the Echinococcus genus, remains a significant zoonotic disease globally. The disease is particularly prevalent in areas...
Understanding ATP binding to DosS catalytic domain with a short ATP-lid
Understanding ATP binding to DosS catalytic domain with a short ATP-lid
ABSTRACTDosS is a heme-sensor histidine kinase that responds to redox-active stimuli in mycobacterial environments by triggering dormancy transformation. Sequence comparison of the...
Targeting the ATP Synthase in Staphylococcus aureus Small Colony Variants, Streptococcus pyogenes and Pathogenic Fungi
Targeting the ATP Synthase in Staphylococcus aureus Small Colony Variants, Streptococcus pyogenes and Pathogenic Fungi
The ATP synthase has been validated as a druggable target with the approval of the ATP synthase inhibitor, bedaquiline, for treatment of drug-resistant Mycobacterium tuberculosis, ...
Spatial control of protein binding with DNA nanostructures
Spatial control of protein binding with DNA nanostructures
<p dir="ltr">The physical and chemical properties of DNA, including its structure predictability thanks to Watson-Crick base pairing, make it into an obvious polymer of choic...
Spatial control of protein binding with DNA nanostructures
Spatial control of protein binding with DNA nanostructures
<p dir="ltr">The physical and chemical properties of DNA, including its structure predictability thanks to Watson-Crick base pairing, make it into an obvious polymer of choic...