Xylopia Aethiopica Suppresses Markers of Oxidative Stress, Inflammation and Cell Death in the Brain of Wistar Rats Exposed to Glyphosate

Abstract Background: The herbicide “Roundup” is used extensively in agriculture to control weeds. However, by translocation, it can be deposited in plants, their proceeds, and in the soil, thus provoking organ toxicities in exposed individuals. Neurotoxicity among others is one of the side effects of roundup which has led to an increasing global concern about the contamination of food by herbicides. Xylopia aethiopicais known to have medicinal properties due to its antioxidative and anti-inflammatory properties, it is hypothesized to neutralize roundup-induced neurotoxicity. Methods: Thirty-six (36) Wistar rats were used for this study. The animals were shared equally into six groups with six rats each. Glyphosate administration to three of the six groups was done orally and for one week. Either Xylopia aethiopicaor vitamin C was co-administered to two of the three groups and also administered to two other groups and the final group served as the control. Results: Our studies demonstrated that glyphosate administration led to a significant decrease in antioxidants such as catalase, superoxide dismutase, glutathione, and glutathione peroxidase. We also observed a significant increase in inflammatory markers such as tumour necrosis factor- α, interleukin 6, C-reactive protein and immunohistochemical expression of caspase-3, cox-2 and p53 proteins (p < 0.05). However, Xylopia aethiopica co-administration with glyphosate was able to ameliorate the aforementioned changes when compared to the control (p < 0.05). Degenerative changes were also observed in the cerebellum, hippocampus, and cerebral cortex upon glyphosate administration. These changes were not observed in the groups treated with Xylopia aethiopica and vitamin C. Conclusion: Taken together, Xylopia aethiopica could possess anti-oxidative and anti-inflammatory properties that could be used in combating glyphosate neurotoxicity.