Phytochemical Screening and Antimicrobial Assessment of Leaves of Adhatoda vasica, Azadirachta indica and Datura stramonium

The present study was aimed to investigate the phytochemical screening and antimicrobial assessment of Adhatoda vasica, Azadirachta indica and Datura stramonium leaf extract. These plants were collected; aqueous and alcoholic extracts were prepared by soxhlet extraction process. The phytochemical analysis of extracts were performed, and antimicrobial activity of aqueous and alcoholic extracts of all plant material at dose of 100 mg/ml and 200 mg/ml concentrations against various strains were done. These extract were studied through agar diffusion method against E. coli, S. aureus, S. Bacillus bacteria and Rhizopus fungi and their comparison with standard ofloxacin. The phytochemical study inferred the presence of secondary metabolites which assist their herbal properties. Azadirachta indica was effective against all of these micro-organisms. While Adhatoda vasica and Datura stramonium were less effective as compared to Azadirachta indica and ineffective against Rhizopus fungi. Azadirachta indica had shown almost equal anti-microbial activity, against all species of microbes which was taken in study as compared to standard ofloxacin. Thus in future, extract of these plants may be beneficial for another several species of microbes and mixture of these plants may be more effective as compared to standard drugs.