Synthesis, Characterization and Evaluation of Cadmium Sulfide Nanoparticles Capped with Dextrin and Functionalized with Temozolomide

Nanoparticles are currently used as drug carriers. Temozolomide is an antineoplastic drug for tumor in central nervous system. It presents a half‐life of 1.8 hours. For this reason, requires high doses that are limited by side effects. Studies about the use of nanoparticles as drugs transporters have reported an increase the drug solubility, increase in half‐life and sustained release of the drug because it is protected from degradation and loss of therapeutic effect. This group has development and studied CdS nanoparticles capped with polymers and have showed being biocompatible and effective as drug carriers. To synthesize and characterize cadmium sulfide nanoparticles capped with dextrin functionalized with temozolomide and observe cell uptake. By means of the chemical method, the precursors were allowed to react to obtain the synthesis of CdS/dextrin nanoparticles. Subsequently, the nanoparticles were functionalized with temozolomide through a chemical method in which the OH groups of the dextrin were bounded to the NH2 Through the technique of Fourier‐transform infrared, the characteristic bonds were observed: at 840 cm−1 the α‐D glucopyranose bond, at 927 cm−1 the α(1–4) bonds; 1159 cm−1 glucopyranosyl units and at 1079 cm−1 the C‐O‐C, at 1025 cm−1 the C‐O bond, at 3399 cm−1 the OH group characteristic of dextrin. At 2001 cm−1 the C=N=N of diazomethane was found which is a toxic compound resulting from the formation of nanoparticles. Also at 3400 cm−1 there is the link N=H and 1610 cm−1 C = C or C = N of the temozolomide. According to the X‐ray results, the widening of 3 peaks corresponding to the crystalline planes characteristic of the cubic phase of the CdS is observed which are: (111), (220) and (311). Using the Scherrer formula d = 0.8λ/βcos θ was calculated that the average size of the nanoparticles is 3 nm. Due to the size of the semiconductor nanoparticles of CdS‐dextrin, they emit green fluorescence characteristic. Therefore, tumor cells treated with nanoparticles functionalized with temozolomide in epifluorescence microscope were observed and it was observed that the nanoparticles reached the cytoplasm and core of cells and produces the effect of temozolomide arresting cell cycle in phase G2 + M. The synthesis and characterization of CdS/dextrin nanoparticles functionalized with temozolomide resulted in 5nm, which are uptake by the cells and are distributed on the cytoplasm and core exerting the effect of the drug. The CdS/dextrin has the potential for being used as drug carriers and facilitating the pharmacological because cells up take them.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.