Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Photosynthetic-product-dependent Activation of Plasma Membrane H + -ATPase and Nitrate Uptake in Arabidopsis Leaves
View through CrossRef
Abstract
Plasma membrane (PM) H
+
-ATPase is a pivotal enzyme for plant growth and development that acts as a primary transporter and is activated by phosphorylation of the penultimate residue, threonine, at the C-terminus. Photosynthetically active radiation activates PM H
+
-ATPase via phosphorylation in mesophyll cells of
Arabidopsis thaliana
, and phosphorylation of PM H
+
-ATPase depends on photosynthesis and photosynthesis-related sugar supplementation, such as sucrose, fructose and glucose. However, the molecular mechanism and the physiological role of photosynthesis-dependent PM H
+
-ATPase activation are still unknown. Analysis using sugar analogues, such as palatinose, turanose, and 2-deoxy glucose, revealed that sucrose metabolites and products of glycolysis such as pyruvate induce phosphorylation of PM H
+
-ATPase. Transcriptome analysis showed that novel isoform of the
Small Auxin Up RNA
genes,
SAUR30
, is upregulated in a light- and sucrose-dependent manner. Time course analyzes of sucrose supplementation showed that phosphorylation level of PM H
+
-ATPase increased within 10 min, but expression level of
SAUR30
increased later than 10 min. The results suggest two temporal regulations may participate in the regulation of PM H
+
-ATPase. Interestingly, a
15
NO
3
-
uptake assay in leaves showed that light increases
15
NO
3
-
uptake, and that increment of
15
NO
3
-
uptake depends on PM H
+
-ATPase activity. The results opened the possibility of physiological role of photosynthesis-dependent PM H
+
-ATPase activation in the uptake of NO
3
-
. We speculate that PM H
+
-ATPase may connect photosynthesis and nitrogen metabolism in leaves.
Title: Photosynthetic-product-dependent Activation of Plasma Membrane H
+
-ATPase and Nitrate Uptake in Arabidopsis Leaves
Description:
Abstract
Plasma membrane (PM) H
+
-ATPase is a pivotal enzyme for plant growth and development that acts as a primary transporter and is activated by phosphorylation of the penultimate residue, threonine, at the C-terminus.
Photosynthetically active radiation activates PM H
+
-ATPase via phosphorylation in mesophyll cells of
Arabidopsis thaliana
, and phosphorylation of PM H
+
-ATPase depends on photosynthesis and photosynthesis-related sugar supplementation, such as sucrose, fructose and glucose.
However, the molecular mechanism and the physiological role of photosynthesis-dependent PM H
+
-ATPase activation are still unknown.
Analysis using sugar analogues, such as palatinose, turanose, and 2-deoxy glucose, revealed that sucrose metabolites and products of glycolysis such as pyruvate induce phosphorylation of PM H
+
-ATPase.
Transcriptome analysis showed that novel isoform of the
Small Auxin Up RNA
genes,
SAUR30
, is upregulated in a light- and sucrose-dependent manner.
Time course analyzes of sucrose supplementation showed that phosphorylation level of PM H
+
-ATPase increased within 10 min, but expression level of
SAUR30
increased later than 10 min.
The results suggest two temporal regulations may participate in the regulation of PM H
+
-ATPase.
Interestingly, a
15
NO
3
-
uptake assay in leaves showed that light increases
15
NO
3
-
uptake, and that increment of
15
NO
3
-
uptake depends on PM H
+
-ATPase activity.
The results opened the possibility of physiological role of photosynthesis-dependent PM H
+
-ATPase activation in the uptake of NO
3
-
.
We speculate that PM H
+
-ATPase may connect photosynthesis and nitrogen metabolism in leaves.
Related Results
V-ATPase Deficiency Aggravates Hypoxia-induced Spermatogenesis Reduction by Promoting Spermatocyte Apoptosis via the JNK/c-Jun Pathway in Mice
V-ATPase Deficiency Aggravates Hypoxia-induced Spermatogenesis Reduction by Promoting Spermatocyte Apoptosis via the JNK/c-Jun Pathway in Mice
Abstract
Spermatocyte apoptosis is the primary cause of poor outcome after hypoxia-triggered spermatogenesis reduction (HSR). The vacuolar H+-ATPase (V-ATPase) has been fou...
Procedure for Western blot v1
Procedure for Western blot v1
Goal: This document has the objective of standardizing the protocol for Western blot. This technique allows the detection of specific proteins separated on polyacrylamide gel and t...
Analysis of canopy light utilization efficiency in high-yielding rapeseed varieties
Analysis of canopy light utilization efficiency in high-yielding rapeseed varieties
Abstract
The photosynthetic mechanism responsible for the differences in yield between different rapeseed varieties remains unclear, and there have been no consensus and de...
Studies of NLP Transcription factors : Roles and mechanisms of NLP-dependent nitrate signaling pathway
Studies of NLP Transcription factors : Roles and mechanisms of NLP-dependent nitrate signaling pathway
Études des facteurs de transcription NLP : Rôles et mécanismes de la voie de signalisation nitrate dépendante des NLPs
L'un des événements moléculaires les mieux ca...
Hierarchy of mechanisms involved in generating Na/K-ATPase polarity in MDCK epithelial cells.
Hierarchy of mechanisms involved in generating Na/K-ATPase polarity in MDCK epithelial cells.
We have studied mechanisms involved in generating a polarized distribution of Na/K-ATPase in the basal-lateral membrane of two clones of MDCK II cells. Both clones exhibit polarize...
An Investigation into Hydrophobic Membrane Fouling in Desalination Using Membrane Distillation Technology
An Investigation into Hydrophobic Membrane Fouling in Desalination Using Membrane Distillation Technology
Demand for freshwater supplies is continuously increasing globally to the extent where some parts of the world became highly water stressed. In particular, the Arabian Gulf states ...
Magnetohydrodynamics enhanced radio blackout mitigation system for spacecraft during planetary entries
Magnetohydrodynamics enhanced radio blackout mitigation system for spacecraft during planetary entries
(English) Spacecraft entering planetary atmospheres are enveloped by a plasma layer with high levels of ionization, caused by the extreme temperatures in the shock layer. The charg...
The function of BoTCP25 in the regulation of leaf development of Chinese kale
The function of BoTCP25 in the regulation of leaf development of Chinese kale
XG Chinese kale (Brassica oleracea cv. ‘XiangGu’) is a variety of Chinese kale and has metamorphic leaves attached to the true leaves. Metamorphic leaves are secondary leaves emerg...