Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Apply Mycoflour kit for detection of Mycoplasma in cell lines at National Institute for Control of Vaccines and Biologicals
View through CrossRef
Control of Mycoplasma in cell culture used in testing is necessary to ensure the accuracy of quality control and vaccine release. Research on fluorescent dyeing process using Mycoplasma detection cell indicators to develop standard operating procedures before routinely used at National Institute for Control of Vaccines and Biologicals (NICVB). Outcome showed that the parameters of the Mycoplasma detection procedure by the Vero cell indicator method using the MycoFlourTM kit at NICVB were optimized for the concentration of Vero cells inoculated into coverslip 10 x104 TB/ml; Wash cells twice with PBS (-)1X and meet the requirements for specificity in cell quality assessment.
National Institute for Control of Vaccines and Biologicals
Title: Apply Mycoflour kit for detection of Mycoplasma in cell lines at National Institute for Control of Vaccines and Biologicals
Description:
Control of Mycoplasma in cell culture used in testing is necessary to ensure the accuracy of quality control and vaccine release.
Research on fluorescent dyeing process using Mycoplasma detection cell indicators to develop standard operating procedures before routinely used at National Institute for Control of Vaccines and Biologicals (NICVB).
Outcome showed that the parameters of the Mycoplasma detection procedure by the Vero cell indicator method using the MycoFlourTM kit at NICVB were optimized for the concentration of Vero cells inoculated into coverslip 10 x104 TB/ml; Wash cells twice with PBS (-)1X and meet the requirements for specificity in cell quality assessment.
Related Results
Transcriptional Changes of Mycoplasma Contamination in Gene Expression Studies
Transcriptional Changes of Mycoplasma Contamination in Gene Expression Studies
Mycoplasma, the smallest self-replicating microorganism, is one of the most significant problems in the cell-culture field. It belongs to the class of Mollicutes, which are charact...
Impact of various preservation and storage methods on the viability of mycoplasma field strains isolated in Mali
Impact of various preservation and storage methods on the viability of mycoplasma field strains isolated in Mali
The survival of five mycoplasma strains was studied in different storage media (mycoplasma complete media without cryopreservative agent, mycoplasma complete media with addition of...
Prevalence of Mycoplasma Infections in Sheep and Goats in Jiangsu, China
Prevalence of Mycoplasma Infections in Sheep and Goats in Jiangsu, China
Background: Mycoplasma infections are widespread globally, causing respiratory and extrapulmonary diseases in animals, particularly in sheep and goats. Symptoms include coughing, n...
Environmental Surveillance Protocols for Highly Pathogenic Avian Influenza (HPAI) v2
Environmental Surveillance Protocols for Highly Pathogenic Avian Influenza (HPAI) v2
EnvironmentalSurveillance Protocols for Highly Pathogenic Avian Influenza (HPAI) This comprehensive protocol suite enables systematic environmental surveillance for avian influenza...
Isolation and identification of mycoplasma strains in the inner ear of cattle and small ruminants in Mali
Isolation and identification of mycoplasma strains in the inner ear of cattle and small ruminants in Mali
Mycoplasmas are microorganisms characterized by the absence of a cell wall and affecting animals and humans. In domestic ruminants, the role of inner ear swabbing was determined in...
Complex Collision Tumors: A Systematic Review
Complex Collision Tumors: A Systematic Review
Abstract
Introduction: A collision tumor consists of two distinct neoplastic components located within the same organ, separated by stromal tissue, without histological intermixing...
Frequency of Common Chromosomal Abnormalities in Patients with Idiopathic Acquired Aplastic Anemia
Frequency of Common Chromosomal Abnormalities in Patients with Idiopathic Acquired Aplastic Anemia
Objective: To determine the frequency of common chromosomal aberrations in local population idiopathic determine the frequency of common chromosomal aberrations in local population...
Data from Neutralization of KIT Oncogenic Signaling in Leukemia with Antibodies Targeting KIT Membrane Proximal Domain 5
Data from Neutralization of KIT Oncogenic Signaling in Leukemia with Antibodies Targeting KIT Membrane Proximal Domain 5
<div>Abstract<p>KIT is a cell surface tyrosine kinase receptor whose ligand stem cell factor (SCF) triggers homodimerization and activation of downstream effector pathw...