Abstract 1546: The research of the mechanism of lung cancer carcinogenesis and the target gene of microRNA-20a in lung cancer small cells

Abstract Purpose: Recent studies have suggested that microRNA biomarkers could be useful for stratifying lung cancer subtypes, but microRNA signatures varied between different populations. Small cell lung cancer (SCLC) is the lowest differentiation degree and the highest malignant subtype of lung cancer that urgently needs biomarkers to aid patient management. Here, we undertook the first comprehensive investigation on microRNA in Chinese SCLC patients of limited stage. Experimental Design: MicroRNA expression was measured in cancerous and noncancerous tissue pairs strictly collected from Chinese SCLC patients of limited stage, who had not been treated with chemotherapy or radiotherapy prior to surgery. The molecular targets of proposed microRNA were further examined. Results: We identified that high expression of hsa-miR-20a was associated with poor survival in these 20 SCLC patients by Kaplan-Meier analysis (P=0.03), This association was independently validated in a separate cohort of 38 SCLC patients in Kaplan-Meier analysis(P=0.046). We then determined that the tumor suppressor PTEN is a target of hsa-miR-20a. Expression of hsa-miR-20a in a human lung cancer cell line repressed PTEN activity. Conclusions: Our results identified a new prognostic biomarker of SCLC, hsa-miR-20a. Note: This abstract was not presented at the meeting. Citation Format: Tan Xiaogang, Hu Mu, Zhi Xiuyi, Jin Shimeng. The research of the mechanism of lung cancer carcinogenesis and the target gene of microRNA-20a in lung cancer small cells. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1546. doi:10.1158/1538-7445.AM2014-1546