Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Expression patterns of T cells-specific long noncoding RNAs in systemic lupus erythematosus patients carrying HLA risk/nonrisk alleles
View through CrossRef
Background Long noncoding RNAs (LncRNAs) play key roles in the regulation of gene expression and subsequently in the pathogenesis of several autoimmune diseases. This study aimed to explore the peripheral expression levels of T-cells-specific LncRNAs and transcription factors in systemic lupus erythematosus (SLE) patients carrying either human leukocyte antigens (HLA) risk or non-risk alleles. Methods Genotypes of HLA-DRB1 and HLA-DQB1 loci for 106 SLE patients were determined by PCR-SSP. In the next step, patients were stratified based on the presence of HLA-DRB1*03 and/or DRB1*16 allele groups (HLA risk alleles positive or HLA-R Pos) or carrying other DRB1 allele groups (HLA-R Neg). Then, transcript levels of LncRNAs ( IFNG-AS1, RMRP, Th2LCR, and DQ786243) and mRNAs for transcription factors ( Foxp3, Gata3, and Tbx21) were measured using qRT-PCR and compared between two subgroups of patients. Results Totally, 47 cases were classified as HLA-R Pos and 59 cases as HLA-R Neg patients. The HLA-R Pos patients showed decreased transcript levels of DQ786243 ( p = .001) and elevated expression of IFNG-AS1 ( p = .06) and T-bet mRNA ( p = .03) compared to the HLA-R Neg group. We observed significantly lower expression of Th2LCR ( p < .0001) and DQ786243 ( p = .001) and higher expression of Tbx21 ( p = .009) and Foxp3 ( p = .02) in DR3-positive versus DR3-negative patients. Likewise, decreased transcript levels of DQ786243 ( p = .02) and RMRP ( p = .003) were observed in DR16-positive versus DR16-negative patients. ROC curve analysis revealed the potential of DQ786243 and RMRP as biomarkers in SLE disease based on the carriage of HLA risk alleles. Conclusions Our results indicate that the contribution of multiple T cell subsets in SLE disease progression as judged by expression analysis of LncRNAs and transcription factors can be inspired by the inheritance of HLA risk/nonrisk alleles is SLE patients.
SAGE Publications
Title: Expression patterns of T cells-specific long noncoding RNAs in systemic lupus erythematosus patients carrying HLA risk/nonrisk alleles
Description:
Background Long noncoding RNAs (LncRNAs) play key roles in the regulation of gene expression and subsequently in the pathogenesis of several autoimmune diseases.
This study aimed to explore the peripheral expression levels of T-cells-specific LncRNAs and transcription factors in systemic lupus erythematosus (SLE) patients carrying either human leukocyte antigens (HLA) risk or non-risk alleles.
Methods Genotypes of HLA-DRB1 and HLA-DQB1 loci for 106 SLE patients were determined by PCR-SSP.
In the next step, patients were stratified based on the presence of HLA-DRB1*03 and/or DRB1*16 allele groups (HLA risk alleles positive or HLA-R Pos) or carrying other DRB1 allele groups (HLA-R Neg).
Then, transcript levels of LncRNAs ( IFNG-AS1, RMRP, Th2LCR, and DQ786243) and mRNAs for transcription factors ( Foxp3, Gata3, and Tbx21) were measured using qRT-PCR and compared between two subgroups of patients.
Results Totally, 47 cases were classified as HLA-R Pos and 59 cases as HLA-R Neg patients.
The HLA-R Pos patients showed decreased transcript levels of DQ786243 ( p = .
001) and elevated expression of IFNG-AS1 ( p = .
06) and T-bet mRNA ( p = .
03) compared to the HLA-R Neg group.
We observed significantly lower expression of Th2LCR ( p < .
0001) and DQ786243 ( p = .
001) and higher expression of Tbx21 ( p = .
009) and Foxp3 ( p = .
02) in DR3-positive versus DR3-negative patients.
Likewise, decreased transcript levels of DQ786243 ( p = .
02) and RMRP ( p = .
003) were observed in DR16-positive versus DR16-negative patients.
ROC curve analysis revealed the potential of DQ786243 and RMRP as biomarkers in SLE disease based on the carriage of HLA risk alleles.
Conclusions Our results indicate that the contribution of multiple T cell subsets in SLE disease progression as judged by expression analysis of LncRNAs and transcription factors can be inspired by the inheritance of HLA risk/nonrisk alleles is SLE patients.
Related Results
Spectrum of cutaneous lupus erythematosus in South Africans with systemic lupus erythematosus
Spectrum of cutaneous lupus erythematosus in South Africans with systemic lupus erythematosus
Background Cutaneous involvement is very common in systemic lupus erythematosus. We describe the prevalence and spectrum of lupus-specific (cutaneous lupus erythematosus) and non-s...
High Resolution HLA-A, HLA-B, and HLA-C Allele Frequencies in Romanian Hematopoietic Stem Cell Donors
High Resolution HLA-A, HLA-B, and HLA-C Allele Frequencies in Romanian Hematopoietic Stem Cell Donors
The HLA genes are associated with various autoimmune pathologies, with the control of the immune response also being significant in organs and cells transplantation. The aim of the...
Contribution of HLA class I (A, B, C) and HLA class II (DRB1, DQA1, DQB1) alleles and haplotypes in exploring ethnic origin of central Tunisians
Contribution of HLA class I (A, B, C) and HLA class II (DRB1, DQA1, DQB1) alleles and haplotypes in exploring ethnic origin of central Tunisians
AbstractBackgroundEstimation of HLA (Human leukocyte Antigen) alleles’ frequencies in populations is essential to explore their ethnic origin. Anthropologic studies of central Tuni...
Diversity of HLA Class I and II Genes in the North Indian Population
Diversity of HLA Class I and II Genes in the North Indian Population
Introduction: Numerous studies have concentrated on specific populations to explore the extensive polymorphism of class I and II HLA genes. This genetic diversity is crucial for va...
Crucial Role of Foxp3 Gene Expression and Mutation in Systemic Lupus Erythematosus, Inferred from Computational and Experimental Approaches
Crucial Role of Foxp3 Gene Expression and Mutation in Systemic Lupus Erythematosus, Inferred from Computational and Experimental Approaches
The impaired suppressive function of regulatory T cells is well-understood in systemic lupus erythematosus. This is likely due to changes in Foxp3 expression that are crucial for r...
HLA Gene Polymorphisms in Romanian Patients with Chronic Lymphocytic Leukemia
HLA Gene Polymorphisms in Romanian Patients with Chronic Lymphocytic Leukemia
Background and Objectives. Numerous genome-wide association studies have highlighted that chronic lymphocytic leukemia (CLL) is a lymphoproliferative disorder with an important gen...
The Impact of Anti-HLA Antibodies on the Prognosis of Allogeneic Hematopoietic Stem Cell Transplantation in Myelodysplastic Syndrome
The Impact of Anti-HLA Antibodies on the Prognosis of Allogeneic Hematopoietic Stem Cell Transplantation in Myelodysplastic Syndrome
Objective To investigate the risk factors of positive anti-HLA antibodies in myelodysplastic syndrome (MDS) patients and gain insights into the impact of anti-HLA antibodies in all...
Next Generation Sequencing Reveals the Association of DRB3*02:02 With Type 1 Diabetes
Next Generation Sequencing Reveals the Association of DRB3*02:02 With Type 1 Diabetes
The primary associations of the HLA class II genes, HLA-DRB1 and HLA-DQB1, and the class I genes, HLA-A and HLA-B, with type 1 diabetes (T1D) are well established. However, the rol...