BG11 medium (working group Wilde) v1

100x BG11 (500 ml) 74.79 g NaNO3 3.75 g MgSO4 × 7H2O 1.8 g CaCl2 × 2H2O 0.30 g Citric Acid 0.28 ml 0,5 M Na2EDTA (pH 8) Add Aqua bidest (ddH2O) to a final volume of 500 ml. Aliquot (5x100 ml) and autoclave. Store at 4°C. Trace-Metal-Mix (500 ml) 1.43 g H3BO3 900 mg MnCl2 × 4H2O 110 mg ZnSO4 × 7H2O 195 mg Na2MoO4 × 2H2O 39.5 mg CuSO4 × 5H2O 24.7 mg Co(NO3)2 × 6H2O Put some ddH2O into the bottle; dissovle the salts seperately and fill up to 500 ml. Sterile filter and aliquot the solution (10 x 50 ml). Store at 4°C. 2x BG11 (1 L) 20 ml 100x BG11 2 ml K2HPO4 × 3H2O (30 mg/ml) 2 ml Na2CO3 (20 mg/ml) 2 ml Trace-Metal-Mix Add 900 ml deionized water, afterwards add the solutions, fill up to 1 L with deionized water and autoclave. Store at RT. Before use add 2 ml of Fe-Ammonium-Citrate (6 mg/ml). 1xBG11 (1 L) 10 ml 100xBG11 10 ml TES pH 8 1 ml K2HPO4 (30 mg/ml) 1 ml Na2CO3 (20 mg/ml) 1 ml Trace-Metal-Mix Add 900 ml deionized water, afterwards add the solutions, fill up to 1 L with deionized water and autoclave. Store at RT. Before use add 1 ml of Fe-Ammonium-Citrate (6 mg/ml).