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Protein Phosphorylation
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Abstract
Reversible phosphorylation is one of the major mechanisms of controlling protein activity in all eukaryotic cells. This new edition of Protein Phosphorylation: A Practical Approach provides a comprehensive description of current methods used to study protein phosphorylation and the kinases and phosphatases which catalyse it. It includes protocols for studying phosphorylation in intact cells; analysis of signal transduction pathways, kinase specificity, and kinase interactions; assay and purification of kinases and phosphatases; and identification of substrates. Also covered are cloning and expression protocols and advice on the crystallization of kinases and phosphatases. Protein Phosphorylation: A Practical Approach 2e will therefore be of great value to any researcher investigating aspects of reversible protein phosphrylation.
Oxford University PressOxford
Title: Protein Phosphorylation
Description:
Abstract
Reversible phosphorylation is one of the major mechanisms of controlling protein activity in all eukaryotic cells.
This new edition of Protein Phosphorylation: A Practical Approach provides a comprehensive description of current methods used to study protein phosphorylation and the kinases and phosphatases which catalyse it.
It includes protocols for studying phosphorylation in intact cells; analysis of signal transduction pathways, kinase specificity, and kinase interactions; assay and purification of kinases and phosphatases; and identification of substrates.
Also covered are cloning and expression protocols and advice on the crystallization of kinases and phosphatases.
Protein Phosphorylation: A Practical Approach 2e will therefore be of great value to any researcher investigating aspects of reversible protein phosphrylation.
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