The Preparation of Functionalized Crosslinked Macroporous Chitosan Microspheres and their Adsorption Properties for Bilirubin

AbstractSummary: glutaraldehyde cross‐linked macroporous chitosan microspheres (CS) were prepared by inverse phase suspension reaction with sugar as porogenic agent. The microspheres were modified with different reagents of 1, 6 hexanediamine (HDA) and low generation polyamidoamine (PAMAM) dendrimers including PAMAM G1.0, PAMAM G2.0, PAMAM G3.0. The content of amino groups on CS, CS‐PAMAM G1.0, CS‐PAMAM G2.0, CS‐PAMAM G3.0, CS‐HDA was 3.56, 5.10, 5.47, 6.47, 4.66 mmol/g, respectively. The bilirubin adsorption on the above five microspheres was carried out in 0.05M phosphate buffer solution (pH = 7.2–7.4) at 37 °C. The results indicated all the modified CS microspheres were better than unmodified CS microspheres for bilirubin adsorption. CS‐HDA has the best adsorption property even if the content of the amino groups was not very high.