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Laboratory Variants GES G170L , GES G170K , and GES G170H Increase Carbapenem Hydrolysis and Confer Resistance to Clavulanic Acid
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The Guiana extended-spectrum (GES) β-lactamase GES
G170H
, GES
G170L
, and GES
G170K
mutants showed
k
cat
,
K
m
, and
k
cat
/
K
m
values very dissimilar to those of GES-1 and GES-5. The enhancement of the hydrolytic activity against carbapenems is potentially due to a shift of the substrate in the active site that provides better positioning of the deacylating water molecule caused by the presence of the imidazole ring of H170 and of the long side chain of K170 and L170.
Title: Laboratory Variants GES
G170L
, GES
G170K
, and GES
G170H
Increase Carbapenem Hydrolysis and Confer Resistance to Clavulanic Acid
Description:
The Guiana extended-spectrum (GES) β-lactamase GES
G170H
, GES
G170L
, and GES
G170K
mutants showed
k
cat
,
K
m
, and
k
cat
/
K
m
values very dissimilar to those of GES-1 and GES-5.
The enhancement of the hydrolytic activity against carbapenems is potentially due to a shift of the substrate in the active site that provides better positioning of the deacylating water molecule caused by the presence of the imidazole ring of H170 and of the long side chain of K170 and L170.
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