GW24-e2354 Effect of Xiongshao Capsule on the platelet activation level and platelet gelsolin in rat model of Acute Myocardial infarction in vivo

Objectives To investigate the effects of active ingredients of Chuanxiong rhizome and red peony root (Chuangxiongol and paeoniflorin) on the platelet activation level and platelet gelsolin in rat model of acute myocardial infarctionin vivo. Methods The rat model of AMI is prepared by ligation of left anterior descending (LAD) artery. Taken Xiongshao Capsule (XSC) as the intervention drug, while aspirin and verapamil (calcium antagonist) as the positive control drugs. Got PRP and PPP by density gradient centrifugation, then gelsolin, F-actin, DBP, P-selection, CK-MB and cTnI were detected by ELISA, the [Ca2+]i of Platelet was detected by FCM, gelsolin expression of infracted myocardium was detected by WB. Results Compare with sham group, the concentration of CK-MB and cTnI of model group increased significantly after ligation of LAD for 3 hours (P < 0.05), and P-selection level increased markedly (P < 0.05). High dose of XSC can reduce CK-MB, cTnI, P-selection, COX-1 and TXB2 level markedly (P < 0.05),which has similar effect with aspirin in vivo. Compare with the sham group, the plasma gelsolin, DBP of Model group reduced significantly (P < 0.05) while platelet gelsolin increased markedly (P < 0.05). High dose of XSC can reduce platelet gelsolin and F-actin (P < 0.05),while increased plasma gelsolin and DBP significantly (P < 0.05). Platelet gelsolin of Model and Xscd group has high positive correlation with platelet activation level (P < 0.05). Compare with sham group,the fluorescence intensity of Ca2+ of model group increased markedly (P < 0.05), and high dose of XSC can inhibit plateletcalcium influx (P < 0.05), which has similar effect of verapamil (P > 0.05). Compare with sham group, the gelsolin expression of infracted myocardium of model group increased markedly, and XSC can inhibit gelsolin expression of infracted myocardium, but verapamil has no such effect. Conclusions The active ingredients of Chuanxiong rhizome and red peony root (Chuangxiongol and paeoniflorin) can reduce the platelet gelsolin level, enhance the activity of EASS, inhibit platelet activation and has the effect of calcium antagonist while it also can inhibit COX-1 and TXB2 which has the similar effect of aspirin in vivo.