Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
To compare and evaluate the efficacy of the groundnut oil and coconut oil with xylene as a clearing agent.
View through CrossRef
Background: Xylene has always been the unmatched clearing medium known to be used tissue processing and staining. However, the toxic effects of “xylene” range from mild irritation to its severe form as a carcinogenic agent. Aims & objective: This study purposes to evaluate the efficacy of “coconut oil” and “groundnut oil” as a substitute in contrast to “xylene” as a clearing agent. Materials and Methods: Each of 40 specimens were fixed and then cut into three equal parts namely; Group A, Group B & Group C. Specimens were cleared in coconut oil, groundnut oil and xylene respectively and all the 3 groups were taken for Haematoxylin and eosin staining (H&E) and Immunohistochemical staining (IHC). Results: Both the oils displayed better results than xylene whereas the coconut oil showed superior results than groundnut oil when checked for gross tissue features, staining quality and cytomorphometric analysis. Conclusion: The results suggest that both coconut oil and groundnut oil has been shown to be an effective replacement for “xylene” as a clearing medium.
Ketaki Prakashan Private Limited
Title: To compare and evaluate the efficacy of the groundnut oil and coconut oil with xylene as a clearing agent.
Description:
Background: Xylene has always been the unmatched clearing medium known to be used tissue processing and staining.
However, the toxic effects of “xylene” range from mild irritation to its severe form as a carcinogenic agent.
Aims & objective: This study purposes to evaluate the efficacy of “coconut oil” and “groundnut oil” as a substitute in contrast to “xylene” as a clearing agent.
Materials and Methods: Each of 40 specimens were fixed and then cut into three equal parts namely; Group A, Group B & Group C.
Specimens were cleared in coconut oil, groundnut oil and xylene respectively and all the 3 groups were taken for Haematoxylin and eosin staining (H&E) and Immunohistochemical staining (IHC).
Results: Both the oils displayed better results than xylene whereas the coconut oil showed superior results than groundnut oil when checked for gross tissue features, staining quality and cytomorphometric analysis.
Conclusion: The results suggest that both coconut oil and groundnut oil has been shown to be an effective replacement for “xylene” as a clearing medium.
Related Results
Characterization and application of bambara groundnut starch-lipid complexes
Characterization and application of bambara groundnut starch-lipid complexes
Bambara groundnut (Vigna subterranea) is an indigenous underutilised leguminous crop to Africa. It is a good source of protein and carbohydrate including starch. Bambara groundnut ...
Influence of Groundnut Populations on Weed Suppression in Cassava-Groundnut Systems
Influence of Groundnut Populations on Weed Suppression in Cassava-Groundnut Systems
<p>Cassava was grown in sole cropping and intercropping with groundnut to determine the performance of associated crops and weed control at three different groundnut populati...
Comparative Study of the Viscosities and Thermal Conductivities of Groundnut and Coconut Oils Dispersed with Graphene Particles Reinforced with Oleic Acid
Comparative Study of the Viscosities and Thermal Conductivities of Groundnut and Coconut Oils Dispersed with Graphene Particles Reinforced with Oleic Acid
This study addresses some challenges accrued using mineral oil as cutting fluid and suggest alternatives to suitable, eco-friendly, non-toxic and biodegradable solution using veget...
Xylenes and Ethylbenzene
Xylenes and Ethylbenzene
AbstractThis article reviews the properties, commercial manufacturing aspects, and uses of the three xylene isomers, ie,o‐xylene,m‐xylene, andp‐xylene, and the ethylbenzene present...
Alternative to xylene as a clearing agent in histopathology
Alternative to xylene as a clearing agent in histopathology
ABSTRACT
INTRODUCTION: Clearing is an essential step in processing tissue for light microscopy. Xylene is the clearing agent used most commonly worldwide. Xylene is toxic...
Penampilan Bibit Kelapa Dalam Bido dan Hibrida Kelapa Genjah x Dalam Bido [Appearance Seed Bido Tall coconut and Hybrids Dwarf x Bido Tall Coconut]
Penampilan Bibit Kelapa Dalam Bido dan Hibrida Kelapa Genjah x Dalam Bido [Appearance Seed Bido Tall coconut and Hybrids Dwarf x Bido Tall Coconut]
<p>Coconut seedlings are growing well with an important role in supporting the success of planting coconut in the field. The higher seed viability are growing well, the more ...
Xylenes and Ethylbenzene
Xylenes and Ethylbenzene
AbstractThis article reviews the properties, commercial manufacturing aspects, and uses of the three xylene isomers, ie,o‐xylene,m‐xylene, andp‐xylene, and the ethylbenzene present...
Revitalization of Economic Development of Coconut Area in North Sulawesi
Revitalization of Economic Development of Coconut Area in North Sulawesi
<p>Coconut is a potential commodity in North Sulawesi Province. At national level, this province contributes of about 9% of the national coconut production, however, the cont...