Search engine for discovering works of Art, research articles, and books related to Art and Culture
ShareThis
Javascript must be enabled to continue!

Effect of Andrographis paniculata polysaccharide on human retinoblastoma Y79 cell proliferation and apoptosis

View through CrossRef
AIM: To explore the effect of the Andrographis paniculata (A. paniculata) polysaccharide on the proliferation and apoptosis of human retinoblastoma (RB) Y79 cells and its mechanism. METHODS: The refined A. paniculata polysaccharide was obtained using techniques such as water extraction, ethanol precipitation, and decompression concentration. The inhibition effect of the A. paniculata polysaccharide on the proliferation of Y79 cells was detected by cell proliferation assay. Flow cytometry was used for the detection of cell apoptosis rate and cycle change. Real-time qunatitative polymerase chain reaction (RT qPCR)and Western blotting were used to detect the expression of cell apoptosis signal pathway-related factors (caspase-3, caspase-8, and caspase-9) and cell cycle signal pathway-related factors (CDK1 and cyclinB1) at the transcriptional and translational levels. RESULTS: Infrared and ultraviolet spectrum scanning showed that the extracted drug was a polysaccharide with high purity. After being treated with different concentrations of A. paniculata polysaccharide for different periods of time, the Y79 cells showed different degrees of proliferation inhibition. Flow cytometric observations showed that the cell apoptosis rate and the proportion of cells blocked in the G2/M phase were significantly increased after A. paniculata polysaccharide treatment. Further analysis revealed that the mRNA and protein expression of caspase-3, caspase-8, and caspase-9 in the A. paniculata polysaccharide treatment groups increased significantly compared with that in the control groups, while the expression of CDK1 and cyclinB1 decreased significantly. CONCLUSION: The A. paniculata polysaccharide could inhibit the proliferation and induce apoptosis of Y79 cells. Its possible mechanism is via the upregulation of caspase-3, caspase-8, and caspase-9 expression in the cell apoptotic signaling pathway and the downregulation of CDK1 and cyclinB1 expression in the cell cycle signaling pathway.
Title: Effect of Andrographis paniculata polysaccharide on human retinoblastoma Y79 cell proliferation and apoptosis
Description:
AIM: To explore the effect of the Andrographis paniculata (A.
paniculata) polysaccharide on the proliferation and apoptosis of human retinoblastoma (RB) Y79 cells and its mechanism.
METHODS: The refined A.
paniculata polysaccharide was obtained using techniques such as water extraction, ethanol precipitation, and decompression concentration.
The inhibition effect of the A.
paniculata polysaccharide on the proliferation of Y79 cells was detected by cell proliferation assay.
Flow cytometry was used for the detection of cell apoptosis rate and cycle change.
Real-time qunatitative polymerase chain reaction (RT qPCR)and Western blotting were used to detect the expression of cell apoptosis signal pathway-related factors (caspase-3, caspase-8, and caspase-9) and cell cycle signal pathway-related factors (CDK1 and cyclinB1) at the transcriptional and translational levels.
RESULTS: Infrared and ultraviolet spectrum scanning showed that the extracted drug was a polysaccharide with high purity.
After being treated with different concentrations of A.
paniculata polysaccharide for different periods of time, the Y79 cells showed different degrees of proliferation inhibition.
Flow cytometric observations showed that the cell apoptosis rate and the proportion of cells blocked in the G2/M phase were significantly increased after A.
paniculata polysaccharide treatment.
Further analysis revealed that the mRNA and protein expression of caspase-3, caspase-8, and caspase-9 in the A.
paniculata polysaccharide treatment groups increased significantly compared with that in the control groups, while the expression of CDK1 and cyclinB1 decreased significantly.
CONCLUSION: The A.
paniculata polysaccharide could inhibit the proliferation and induce apoptosis of Y79 cells.
Its possible mechanism is via the upregulation of caspase-3, caspase-8, and caspase-9 expression in the cell apoptotic signaling pathway and the downregulation of CDK1 and cyclinB1 expression in the cell cycle signaling pathway.

Related Results

Abstract POSTER-THER-1407: Cytotoxic natural human MAb against ovarian carcinoma
Abstract POSTER-THER-1407: Cytotoxic natural human MAb against ovarian carcinoma
Abstract MAb216 is a human IgM mAb encoded in germline configuration by the immunoglobulin heavy chain VH4-34 gene. This antibody and many others derived from the VH...
Genetic interaction mapping highlights key roles of the Tol-Pal complex
Genetic interaction mapping highlights key roles of the Tol-Pal complex
AbstractThe conserved Tol-Pal trans-envelope complex is important for outer membrane (OM) stability and cell division in Gram-negative bacteria. It has been proposed to mediate OM ...
Abstract POSTER-BIOL-1352: Notch3 promotes anoikis resistance in ovarian cancer
Abstract POSTER-BIOL-1352: Notch3 promotes anoikis resistance in ovarian cancer
Abstract Epithelial ovarian cancer (EOC) is the most lethal gynecologic cancer. Recently, a number of genome-wide studies in ovarian cancer patient samples have post...
Abstract POSTER-BIOL-1314: Investigating fatty acid beta-oxidation in ovarian cancer cells
Abstract POSTER-BIOL-1314: Investigating fatty acid beta-oxidation in ovarian cancer cells
Abstract Purpose: To investigate the contribution of fatty acid beta-oxidation to the total energy budget of ovarian cancer cells. Experimental proced...
Self-Organization of Symmetry Networks: Transformation Invariance from the Spontaneous Symmetry-Breaking Mechanism
Self-Organization of Symmetry Networks: Transformation Invariance from the Spontaneous Symmetry-Breaking Mechanism
Symmetry networks use permutation symmetries among synaptic weights to achieve transformation-invariant response. This article proposes a generic mechanism by which such symmetries...
Toward Net Zero: an Engine Electrification Strategy Approach of Fuel Cell and Steam Injection
Toward Net Zero: an Engine Electrification Strategy Approach of Fuel Cell and Steam Injection
Abstract The turbofan engine electrification is a promising element in the global effort to achieve the 2050 net-zero emission target. This transformative shift embr...
Cancer immunotherapy: potential involvement of mediators
Cancer immunotherapy: potential involvement of mediators
The description of a cell‐free soluble anti‐tumour factor by Carswell et al. in 1975 (Proc Natl Acad Sci USA, 72: 3666–3670) was followed by a long series of experimental and clini...
Advances in the research of plant-derived natural products against retinoblastoma
Advances in the research of plant-derived natural products against retinoblastoma
Retinoblastoma (RB) is a highly aggressive ocular tumor, and due to socioeconomic and medical constraints, many children receive treatment only in the metaphase and advanced clinic...

Recent Results

CATnews - Rapid Impact Modelling of Earthquakes for Social Media in the 2020s
CATnews - Rapid Impact Modelling of Earthquakes for Social Media in the 2020s
<p>The public attention to natural disasters increased significantly over the last couple of years. To some extend this can be linked to an increased activity of soci...
Vergence Dynamics Predict Fixation Disparity
Vergence Dynamics Predict Fixation Disparity
The neural origin of the steady-state vergence eye movement error, called binocular fixation disparity, is not well understood. Further, there has been no study that quantitatively...
Enhancing heart disease diagnosis through precise heart sound including infrasound
Enhancing heart disease diagnosis through precise heart sound including infrasound
Recent research has been pushing the frontier of diagnosing cardiovascular diseases, which have a high mortality rate globally. The need for swift responses to acute diseases has u...

Back to Top