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Presence of phytoplasma in Chaya an ancient Maya edible plant
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Abstract Cnidoscolus aconitifolius ssp. aconitifolius and C. souzae are shrubs that are widely distributed in the Yucatan Peninsula, Mexico. In recent years, they have shown symptoms characteristic of phytoplasma infection. Therefore, they were screened for the presence of phytoplasma using nested PCR with P1 and P7 primers, followed by R16F2n/R16R2 primers for universal phytoplasma detection. Through this protocol, phytoplasma was detected in both species that exhibited symptoms of infection. Sequence analysis, virtual RFLPs, and a phylogenetic tree confirmed that the Cnidoscolus species analyzed and sequenced are infected by phytoplasmas similar to those that infect cassava, belonging to subgroups 16SrIII-L. This study is the first to report the presence of phytoplasmas in chaya species.
FapUNIFESP (SciELO)
Title: Presence of phytoplasma in Chaya an ancient Maya edible plant
Description:
Abstract Cnidoscolus aconitifolius ssp.
aconitifolius and C.
souzae are shrubs that are widely distributed in the Yucatan Peninsula, Mexico.
In recent years, they have shown symptoms characteristic of phytoplasma infection.
Therefore, they were screened for the presence of phytoplasma using nested PCR with P1 and P7 primers, followed by R16F2n/R16R2 primers for universal phytoplasma detection.
Through this protocol, phytoplasma was detected in both species that exhibited symptoms of infection.
Sequence analysis, virtual RFLPs, and a phylogenetic tree confirmed that the Cnidoscolus species analyzed and sequenced are infected by phytoplasmas similar to those that infect cassava, belonging to subgroups 16SrIII-L.
This study is the first to report the presence of phytoplasmas in chaya species.
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