Flow Cytometry for Evaluation of EGFR-mTOR Signal Way in the Growth Inhibitory Rate of Hela Cell Under Low Glucose and High Lactic Acid Environment

Objective: To discuss the effect of EGFR-mTOR signal way in the adaption of cervical carcinoma cell in low glucose and high lactic acid. Methods: Hela cells were cultured in four conditions: normal glucose (NG, glucose 10 mmol/L), low glucose (LG, glucose 3 mmol/L), high lactic acid (HL, lactic acid 2.5 mmol/L) and low glucose and high lactic acid (LGHL, glucose 10 mmol/L, lactic acid 2.5 mmol/L). Growth inhibitory rate of Hela cell was determined by CCK-8; Flow cytometry (FCM) was performed to evaluate the cell cycle; Results: Compared with the rate in NG, the rate in low glucose and high lactic acid was significantly increased (P < 0.01). Compared with the rate in NG, the rate of G1/G0 was increased in HL and in LGHL (P < 0.01, P < 0.01); the rate of G1/G0 in LG didn't have significant difference; the rates of G2/M in HL and in LGHL were decreased, and the G2/M rate in LG was increased, but all of them had no statistically significant. The rates of S were decreased in HL, LG and LGHL (P < 0.05, no statistically significant, P < 0.05). The expression level of EGFR mRNA in Hela was decreased and the level of mTOR mRNA was increased in HL (both had no statistically significant), when compared with the levels in NG. The expression level of EGFR mRNA was decreased (P < 0.05) and the level of mTOR mRNA was a little increased (no statistically significant) in LG; while the EGFR and mTOR expression levels were increased in LGHL (P < 0.05). Conclusion: Low glucose and high lactic acid environment is conducive to Hela cell survival, and can promote the expression of EGFR and mTOR.