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Two different aldolase A mRNA species in rat tissues
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Double‐stranded DNA was synthesized with reverse transcriptase from size‐fractionated poly(A)‐containing RNA from rat ascites hepatoma cells. The cDNA was introduced into Escherichia coli HB101 using pBR 322 DNA as a cloning vector. Several plasmids containing aldolase A cDNA were identified by colony hybridization with 32P‐labeled cDNA prepared from immunologically purified aldolase A mRNA. The partial amino acid sequence of the cDNA sequence was determined, and found to coincide with that of rabbit aldolase A.Using aldolase A cDNA as a hybridization probe, the aldolase A mRNA concentrations in various rat tissues were analysed, and two aldolase A mRNA species differing in nucleotide length were found; the smaller mRNA (about 1550 nucleotides) in muscle, and the larger one (about 1650 nucleotides) in brain and hepatoma cells.
Title: Two different aldolase A mRNA species in rat tissues
Description:
Double‐stranded DNA was synthesized with reverse transcriptase from size‐fractionated poly(A)‐containing RNA from rat ascites hepatoma cells.
The cDNA was introduced into Escherichia coli HB101 using pBR 322 DNA as a cloning vector.
Several plasmids containing aldolase A cDNA were identified by colony hybridization with 32P‐labeled cDNA prepared from immunologically purified aldolase A mRNA.
The partial amino acid sequence of the cDNA sequence was determined, and found to coincide with that of rabbit aldolase A.
Using aldolase A cDNA as a hybridization probe, the aldolase A mRNA concentrations in various rat tissues were analysed, and two aldolase A mRNA species differing in nucleotide length were found; the smaller mRNA (about 1550 nucleotides) in muscle, and the larger one (about 1650 nucleotides) in brain and hepatoma cells.
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