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Target factor analysis: UV spectrophotometry for the simultaneous determination of six amino acids

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AbstractThe UV spectra of mixture solutions consisting of tyrosine, tryptophane, phenylalanine, cystine, histidine and 3,4‐dihydroxyl phenylalanine have been measured. The numbers, identities and concentrations of the amino acids in the mixtures have been determined successfully using target factor analysis. The effects of the wavelength range and the selected sampling interval on the results are discussed. Twenty‐five synthetic mixture samples have been analysed successfully. The average recoveries are 98.9 for Tyr, 96.5 for Trp, 105.6 for Phe, 98.1 for Cys, 98.9 for His and 106.4 for Di‐phe. The results obtained are in good agreement with those obtained by the Kalman filter method.
Title: Target factor analysis: UV spectrophotometry for the simultaneous determination of six amino acids
Description:
AbstractThe UV spectra of mixture solutions consisting of tyrosine, tryptophane, phenylalanine, cystine, histidine and 3,4‐dihydroxyl phenylalanine have been measured.
The numbers, identities and concentrations of the amino acids in the mixtures have been determined successfully using target factor analysis.
The effects of the wavelength range and the selected sampling interval on the results are discussed.
Twenty‐five synthetic mixture samples have been analysed successfully.
The average recoveries are 98.
9 for Tyr, 96.
5 for Trp, 105.
6 for Phe, 98.
1 for Cys, 98.
9 for His and 106.
4 for Di‐phe.
The results obtained are in good agreement with those obtained by the Kalman filter method.

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