Antigenic and immunogenic properties of industrial strains of Porcine Teschovirus

90 samples of brain and spinal cord from dead or forced slaughtered pigs with clinical signs Teschen disease were selected and investigated. Of the 28 samples taken we selected isolates viruses which have morphological, physical, chemical, biological, molecular-genetic and antigenic properties attributed to species Porcine teschovirus (PTV) genus Teschovirus of the family Picornaviridae. According to the results of previous screening for further studies 4 strains were selected. In the neutralization test of the virus from hyperimmune rabbit sera to the reference strains of Porcine teschovirus, Porcine sapelovirus and Enterovirus G we found that the strains of Dniprovsky-34, Dniprovsky-33, Dniprovsky-34 belonging to PTV-1, strain Gorodnyansky-31 were neutralized serum to PTV 1, 10 and 11 serotypes. Determination of affinity and antigenic differences of these strains among themselves and with other epizootic and production strains found that the most extensive links with antigenic strains Gorodnyansky-31 and Dniprovsky34. A study of the immunogenicity of strains Dniprovsky-34 and Gorodnyansky-31 found that virus neutralizing antibodies in the blood serum of animals in credits 1:128-1:2048 is stored for six months. Accordingly, the strain Dniprovsky-34 was selected for the production of diagnostic and specific prevention Teschen disease, and strain of Gorodnyansky-31 — for the diagnostic and prevention teshoviruses encephalomyelitis, etiological agents which may be Porcine teschovirus of other serotypes.