Chelator production by Deschampsia cespitosa (L.) Beauv. in adaptive Ni/Cu hyper-tolerance derived from fields in the Sudbury region and lab assessment

Plants possess a complex network of mechanisms to utilize and, if necessary, detoxify metals. Plants utilize constitutive basal tolerance mechanisms to maintain appropriate internal metal levels under normal conditions. However, adaptive hyper-tolerance mechanisms are used in order to tolerate excess metal exposure. The production of metal binding chelators could be one way to convey these tolerances. Chelator production of field and greenhouse-derived materials was investigated to determine any multi-metal hyper-tolerances in different populations of the grass Deschampsia cespitosa (L.) Beauv. Plant tissue was collected from metal-contaminated mine sites, and from specimens grown in metal exposure hydroponic experiments. The chelator metabolites from these samples were simultaneously analyzed using HPLC-tandem mass spectrometry. In the hydroponic grown grass, histidine was produced at high concentrations solely in the hyper-tolerant populations during metal exposure. In all of the populations, the responses of chelators were metal-specific, where levels of nicotianamine were at high concentrations during Ni exposure, and levels of phytochelatins were high during Cu exposure. Moreover, a similar pattern of chelator production was seen in the root specimens collected from mine sites contaminated with Ni and (or) Cu. Histidine was the strongest Ni chelator involved in adaptive hyper-tolerance, while constitutive basal tolerance to Ni and Cu was observed via the responses of nicotianamine and phytochelatin, respectively.