Culture optimization and regulation mechanism of sporosarcina pasteurii for restoring root splitting fissures in Jinyang ancient city

To master the culture optimization and regulation mechanism of Sporosarcina Pasteurii, this study explored the adaptability of MICP technology for restoring root splitting fissures of plants, and used single factor comparative experimental method to study the characteristics of growth curves and urease activity of Pasteurii in different conditions in earthen sites of Jinyang Ancient City. The conclusions are as follows. The technology of MICP was an ideal method for restoring root splitting fissures of plants, which could meet the requirements of restoring earthen sites by sealing surface fissures, inhibiting root growth of plants and preserving the physical morphology of damaged plant roots and stems. It was recommended to use the medium of M1, with an initial pH of 7.25, a culture temperature of 30 , inoculation age range of 12-13h, and inoculation amount of 3%. The suggestion was to select Pasteurii as restoration solution when the urease activity was at peak period. The conclusions can provide suitable breeding conditions for Pasteurii, providing an important reference for MICP technology to restore root splitting fissures of plants for earthen sites.