Receptor Identification and Characterization

Title: Receptor Identification and Characterization

Description:

AbstractThe sections in this article are:Identification of Receptors Based on Functional PropertiesSchild AnalysisIdentification of Receptors with Radioligand BindingChoosing the Biological PreparationChoosing a RadioligandSeparating Bound From Free RadioligandIrrelevant BindingEstablishing That the Radioligand Binding Detected Reflects Interaction With the Physiologically Significant ReceptorAnalysis of Radioligand Binding Data Based on the Law of Mass ActionFractional OccupancyAssumptions Inherent in the Law of Mass ActionSaturation Binding StudiesDefining Nonspecific Binding in a Radioligand Binding AssayDefining The Specificity of Radioligand Binding Using Competitive Binding AnalysisAnalyzing Competitive Binding DataCalculating the KiFrom the IC50Homologous Competitive Binding Curves Permit an Assessment of Both Kdand BmaxKinetic Analysis of Radioligand Binding ExperimentsStudies of Radioligand DissociationStudies of Radioligand AssociationComplex Binding PhenomenaCompetitive Binding Experiments With Two (or More) Receptor SitesSaturation Binding Experiments With Two Receptor SitesComparing One‐ and Two‐Site ModelsThe Slope Factor of a Competitive Binding CurveUsing Dissociation Experiments to Investigate Complex BindingDistinguishing Between Independent Receptor Subtypes and Negative CooperativityEvaluating Allosteric PhenomenaObtaining Independent Data to Clarify the Biological Origin of Complex Binding PhenomenaAgonist Binding and the Ternary ComplexSummary.