Impact of licorice supplementation on cardiac biomarkers and histomorphological changes in rats

Abstract Objectives This study aimed to evaluate the impact of oral licorice supplementation on cardiotoxic biomarkers and histological changes in cardiac tissue in rats, given the widespread use of licorice (Glycyrrhiza glabra) for its anti-inflammatory, antioxidant, and antimicrobial properties and the concerns about its cardiotoxic effects at higher doses or with short-term repeated use. Methods Twenty-four female Sprague-Dawley rats were divided into four groups (n=6 per group). Groups received either distilled water or licorice extract at 50, 100, and 200 mg/kg/day for 14 days. Cardiac tissue was analyzed via H&E staining, and blood samples were assessed for Troponin-T and Pro-BNP levels. Results No significant changes were observed in Troponin-T and Pro-BNP levels across all groups (p>0.05). Histological analysis revealed mild changes in the cardiac tissues of rats treated with licorice, indicating subtle histomorphological alterations. Conclusions Licorice supplementation at doses of 50, 100, and 200 mg/kg/day did not significantly impact the levels of cardiotoxic biomarkers but mild histomorphological changes were observed in the cardiac tissues of rats. These findings suggest that while licorice is generally safe at these doses, its long-term use at high doses should be approached with caution.