Optimiser™: The next generation of microplates (144.18)

Abstract The Optimiser™ microplates combine the power of microfluidics with the 96-well architecture to offer unparalleled performance. The Optimiser consists of an array of wells defined on one face of a substrate connected to microfluidic channels on the opposing face. The channels are sealed by a sealing layer with opening at outlet of the channel, which in turn connects to an absorbent pad. In the Optimiser the microfluidic channel (with a 50% higher surface area and 50x higher surface area to volume ratio) serves as the reaction chamber, allowing for low volume, rapid assay reactions. During use, the first reagent is added to the well, drawn into the channel by capillary forces and transported to the absorbent pad and partially sucked out. The unique design allows the liquid to exit the well but be trapped within the channel for static incubation. Further steps only involve sequential reagent additions which are transported through the channel for a complete assay sequence. The Optimiser has been successfully tested in chemifluorescence mode to demonstrate comparable sensitivity to a 96-well plate with < 1/3rd sample volume. Alternately with similar sample volumes, the Optimiser boasts >10x higher sensitivity, to achieve detection limits of < 1 pg from a 100 µl sample. In all cases, a complete sandwich assay can be completed in < 30 minutes with the Optimiser. The Optimiser conforms to ANSI microplate specs and can be used with all current microplate instruments.