Association of Apa1 Vitamin D Receptor Polymorphism and Clinicopthological Characteristics of Breast Cancer at Sanglah Hospital Denpasar Bali

Introduction: Cancer is one of the diseases associated with aging. Breast cancer is one of the most common cancers in women. There are many studies linking vitamin D receptor expression with breast cancer. Many previous studies have shown a significant association between the Apa1 vitamin D receptor and the clinicopathological characteristics of breast cancer. This study aims to prove the association between the Apa1 vitamin D receptor polymorphism and the clinicopathological characteristics of breast cancer at Sanglah Hospital, Denpasar Bali. Methods: This research is an analytic study with cross sectional method. The research was conducted at the Integrated Biomedical Laboratory Unit of the Faculty of Medicine, Udayana University from February to May 2021. The samples in this study were 38 stored biological materials in the form of DNA from breast cancer patients in the Biochemistry Laboratory of the Udayana Faculty of Medicine. To determine the polymorphism of the Apa1 vitamin D receptor, DNA samples from breast cancer patients were amplified by polymerase chain reaction (PCR) method, then identified by sequencing method. Statistical analysis used bivariate and multivariate analysis to determine the association between Apa1 vitamin D receptor polymorphism and clinicopathological characteristics of breast cancer.  Result: From the sequencing results, it was found that there were 14 people (36.8%) with homozygous Wildtype CC, 7 people (18.4%) with heterozygous AC, and 17 people (44.7%) with homozygous polymorphism AA. There was no significant association between age, menopausal status, and history of contraception with breast cancer clinicopathology, but menopausal status was significant with the stage of breast cancer patients (p=0.046). There was no significant association between the Apa1 vitamin D receptor polymorphism with the clinicopathological characteristics of breast cancer where the p value> 0.05. Conclusion: There was no significant association between the Apa1 vitamin D receptor polymorphism with the clinicopathological characteristics of breast cancer at Sanglah Hospital, Denpasar Bali.