Microbial 1,2-dehydrogenation of 6 Alpha-Methylhydrocortisone 11-trifluoroacetate

Transformation of 11-trifluoroacetate 6α-methylhydrocortisone (11-TFA MHC) by cells of actinobacteria Arthrobacter (Nocardioides) was carried out in the presence of α-cyclodextrin (α-CD). The composition and dynamics of the transformation products accumulation in the culture medium at various pH values and the ratio of α-CD/substrate were studied. It was shown that the addition of α-CD to the transformation medium at pH less than 7 promotes an increase in the rate of 1,2-dehydrogenation with the formation of 6 α-methylprednisolone 11-trifluoroacetate (11-TFA MPL). At pH over 7, the primary process is the hydrolysis of the 11β-trifluoroacetyloxy group. In this case, the participation of α-CD in these processes as an acceptor of the trifluoroacetyl ion is not excluded.