Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Molecular analysis of the aroA gene of Pasteurella multocida and vaccine potential of a constructed aroA mutant
View through CrossRef
SummaryThe aroA gene from Pasteurella multocida was cloned by complementation of the Escherichia coli aroA mutant AB2829 with a DNA library constructed in pUC18. The nucleotide sequence of the P. multocida aroA gene indicated an open reading frame encoding a protein of 441 amino acids, which showed a high degree of homology with the amino acid sequences of various other bacterial AroA proteins. The cloned P. multocida aroA gene was inactivated by insertion of a kanamycin‐resistance gene and reintroduced by allelic exchange into the chromosome of P. multocida using the suicide vector pJM703.1. The P. multocida aroA mutant was highly attenuated in a mouse model. Mice immunized intraperitoneally with two doses of live P. multocida aroA mutant were completely protected against a lethal parental strain challenge.
Title: Molecular analysis of the aroA gene of Pasteurella multocida and vaccine potential of a constructed aroA mutant
Description:
SummaryThe aroA gene from Pasteurella multocida was cloned by complementation of the Escherichia coli aroA mutant AB2829 with a DNA library constructed in pUC18.
The nucleotide sequence of the P.
multocida aroA gene indicated an open reading frame encoding a protein of 441 amino acids, which showed a high degree of homology with the amino acid sequences of various other bacterial AroA proteins.
The cloned P.
multocida aroA gene was inactivated by insertion of a kanamycin‐resistance gene and reintroduced by allelic exchange into the chromosome of P.
multocida using the suicide vector pJM703.
1.
The P.
multocida aroA mutant was highly attenuated in a mouse model.
Mice immunized intraperitoneally with two doses of live P.
multocida aroA mutant were completely protected against a lethal parental strain challenge.
Related Results
Identification of serotypes of Mannheimia haemolytica and Pasteurella multocida from pneumonic cases of sheep and goats and their antimicrobial sensitivity profiles in Borana and Arsi zones, Ethiopia
Identification of serotypes of Mannheimia haemolytica and Pasteurella multocida from pneumonic cases of sheep and goats and their antimicrobial sensitivity profiles in Borana and Arsi zones, Ethiopia
AbstractRespiratory diseases caused by Mannheimia haemolytica (M. haemolytica) and Pasteurella multocida (P. multocida) have been known to result in a considerable loss due to mort...
Isolation and Identification of Pasteurella multocida and Mannheim Hemolytica from Pneumonic Small Ruminants in Haramaya District, Eastern Ethiopia
Isolation and Identification of Pasteurella multocida and Mannheim Hemolytica from Pneumonic Small Ruminants in Haramaya District, Eastern Ethiopia
Abstract
Background
Pasteurella species are frequently encountered as serious pathogens in small ruminants.
Methods
The cross-sectional study was conducted from December ...
Identification of Serotypes of Mannheimia haemolytica and Pasteurella multocida from pneumonic cases of sheep and goats and their antimicrobial sensitivity profiles in Borana and Arsi zones, Ethiopia
Identification of Serotypes of Mannheimia haemolytica and Pasteurella multocida from pneumonic cases of sheep and goats and their antimicrobial sensitivity profiles in Borana and Arsi zones, Ethiopia
Abstract
Respiratory diseases caused by M. haemolytica and P. multocida have been known to result in a considerable loss due to mortality and reduced production. This study...
Molecular characteristic of Pasteurella multocida isolates from Sumba Island at East Nusa Tenggara Province, Indonesia
Molecular characteristic of Pasteurella multocida isolates from Sumba Island at East Nusa Tenggara Province, Indonesia
Aim: This study aimed to determine the molecular characteristics of Pasteurella multocida isolates originated from Sumba Island, East Nusa Tenggara Province.
Materials and Methods...
Identification of serotypes Pasteurella multocida and Mannheimia haemolytica from cattle and sheep in central Ethiopia
Identification of serotypes Pasteurella multocida and Mannheimia haemolytica from cattle and sheep in central Ethiopia
Abstract
Background: Pneumonic pasteurellosis is a multi-factorial respiratory disease of cattle and sheep caused by combination of etiologic agents; hence, reliable inform...
Infection of lung cavitations in a young dog owner with Hodgkin’s lymphoma caused by Pasteurella multocida, without a dog bite: confirmed zoonotic transmission by tagmentation microbiome analysis
Infection of lung cavitations in a young dog owner with Hodgkin’s lymphoma caused by Pasteurella multocida, without a dog bite: confirmed zoonotic transmission by tagmentation microbiome analysis
Pasteurella multocida is a known pathogen in humans, mostly reported after animal bite incidents. Atraumatic infections have been described, especially in immunocompromised patient...
Culture Negative Pasteurella multocida Confirmed Prosthetic Hip Infection using Next-generation Sequencing
Culture Negative Pasteurella multocida Confirmed Prosthetic Hip Infection using Next-generation Sequencing
Introduction: Prosthetic joint infections (PJIs) are a dreaded complication of joint arthroplasty. Zoonotic organisms such as Pasteurella multocida (PM) rarely cause PJIs. Still, t...
Pasteurella multocida Endocarditis with Septic Arthritis: Case Report and Review of the Literature
Pasteurella multocida Endocarditis with Septic Arthritis: Case Report and Review of the Literature
Background: There is a paucity of evidence regarding optimal management of Pasteurella spp. endocarditis. The authors report the first case of Pasteurella spp. endocarditis with se...