Validating the properties of Ranunculus sceleratus Linn. by performing spectroscopic techniques and modern chromatography

Ranunculus sceleratus Linn. is one of the well-known medicinal plants, being used from the ancient time in India and commonly called as “Jal dhaniya”. It belongs to Ranunculaceae family derived from two Latin Words “Rana” means frog and “unculus” means little and referred together as “little frog” and also species sceleratus for cursed. It is an aquatic perennial herb. It consists of a herbaceous hollow stem, firm tap root, branched rhizome and leaves having a smooth upper side. The green plant is toxic for livestock and uncomfortable to human skin. This plant can grow up to 0.60 m tall, and also used as a food, medicine and possess other uses. Ranunculus sceleratus Linn. is commonly spread in the temperate and cold region in Global distribution (Indonesia, Malaysia, Nepal, Sri Lanka and India). According to Bentham and Hooker classification “Genera Plantarum”, this plant belongs to the division Polypetale of Dicotyledones which processes more than 600 species. The Phytochemical screening was performed according to API norms, in addition to this UV VIS, FTIR, TLC and HPLC test were also carried out for further validation. The spectroscopy and chromatography examination revealed the presence of flavonoids, phenols and various other Phytoconstituents in Ranunculus sceleratus Linn. The spectroscopy and chromatography validation can help us it for medicinal and commercial purposes.