Upregulation of CD271 Transcriptome in Breast Cancer Promotes Cell Survival via NFκB Pathway

Abstract BackgroundBiological treatment of many cancers currently targets membrane bound receptors located on a cell surface. We are in a great to need identify novel membrane proteins associated with migration and metastasis of breast cancer cells. CD271, a single transmembrane protein belongs to tumor necrosis factor receptor acts and play its role in proliferation of cancer cell. The purpose of this study is to investigate the role of CD271 in breast cancer. Methods and ResultsIn this study we analyzed the expression of CD271 in breast tumor tissue, breast cancer cell line MCF7 and isolated cancer stem cells (MCF7-CSCs) by quantitative real-time polymerase chain reaction (RT-qPCR). CD271 was upregulated among breast cancer patients in all age groups. Within the promoter region of CD271, there is a binding site for NF-κB1 which overlaps a putative quadraplex forming sequence. While CD271 also activates NF-κB pathway, down regulation of CD271 through quadraplex targeting resulted in inhibition of NF-κB and its downstream targets Nanog and Sox2ConclusionIn conclusion, CD271 and NF-κB are interrelated to each other. Upon CD271 inhibition, the NF-κB expression also reduces which then effected the cell proliferation and migration. These results suggest that NF-κB is regulated by CD271 is playing a crucial role in cancer development and could be a potential therapeutic target.