Proteomics Analysis and Identification of Critical Proteins and Network Interactions That Regulate the Specific Deposition of IMF of Jingyuan Chicken

Abstract Background:Improving the production efficiency and quality of broiler chickens has become a hot topic in molecular breeding of the broiler industry. There are several factors, among which intramuscular fat (IMF) content is one of the essential factors determining chicken quality. The chicken carcass weight of 40-50% consisted of the leg muscles and breast muscles, and the research on chicken meat quality was performed on that part. Jingyuan chicken is one of the high-quality local chicken species in China. However, the molecular mechanism of IMF specific deposition in different parts of Jingyuan chicken is still unclear. In order to screen and identify the proteins that regulate IMF specific deposition in different parts of Jingyuan chicken and analyze network interaction, the present study selected 180-day-old Jingyuan chicken as the research material, using proteomic techniques and PRM experimental methods to carry out basic research on leg muscle and breast muscle. Results:Three key signaling pathways and ten essential functional proteins regulating IMF specific deposition of Jingyuan chicken were identified, while the molecular mechanism of the deposition of proteins was explained, and analyzed the network interaction between these key functional proteins. Conclusions:The results of this study are of great scientific value and theoretical significance not only for improving chicken quality but also for molecular breeding of high-quality local chickens.