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IN VITRO EFFICACY OF PGPR PSEUDOMONAS FLUORESCENS AGAINST FUSARIUM WILT OF TOMATO CAUSED BY FUSARIUM OXYSPORUM F.SP. LYCOPERSICI
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Tomato (Solanum lycopersicum L.) is one of the most important, commercial and widely grown vegetable crop in the world. Tomato plays a critical role in nutritional food requirements, income and employment opportunities for the people. However, its production is threatened by the Fusarium wilt caused by Fusarium oxysporum f.sp. lycopersici and production losses between 30%to40%. In the present investigation an attempt has been made to study the in vitro efficacy of Pseudomonas fluorescens against Fusarium oxysporum f.sp. lycopersici. The antagonistic effect of Pseudomonas fluorescens were observed by the Dual culture technique and Agarwell method under the in vitro conditions.Among the ten isolates of Pseudomonas fluorescens, isolate Pf5 found to show the maximum percent inhibition over control (58.75%) and least mycelial growth (37.12mm) in dual culture technique against Fusarium oxysporum f.sp. lycopersici. In Agar well method isolate Pf5 proved out the maximum inhibition zone (17.47mm)against Fusarium oxysporumf.sp. lycopersici and percent inhibition over control (80.97%) at 30% concentration level.
Title: IN VITRO EFFICACY OF PGPR PSEUDOMONAS FLUORESCENS AGAINST FUSARIUM WILT OF TOMATO CAUSED BY FUSARIUM OXYSPORUM F.SP. LYCOPERSICI
Description:
Tomato (Solanum lycopersicum L.
) is one of the most important, commercial and widely grown vegetable crop in the world.
Tomato plays a critical role in nutritional food requirements, income and employment opportunities for the people.
However, its production is threatened by the Fusarium wilt caused by Fusarium oxysporum f.
sp.
lycopersici and production losses between 30%to40%.
In the present investigation an attempt has been made to study the in vitro efficacy of Pseudomonas fluorescens against Fusarium oxysporum f.
sp.
lycopersici.
The antagonistic effect of Pseudomonas fluorescens were observed by the Dual culture technique and Agarwell method under the in vitro conditions.
Among the ten isolates of Pseudomonas fluorescens, isolate Pf5 found to show the maximum percent inhibition over control (58.
75%) and least mycelial growth (37.
12mm) in dual culture technique against Fusarium oxysporum f.
sp.
lycopersici.
In Agar well method isolate Pf5 proved out the maximum inhibition zone (17.
47mm)against Fusarium oxysporumf.
sp.
lycopersici and percent inhibition over control (80.
97%) at 30% concentration level.
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