The Neuroprotective Effects of Ocimum gratissimum- Supplemented Diet on Scopolamine-Induced Memory Impairment in Mice Model of Alzheimer’s Disease

ABSTRACT Background Cognitive decline is a hallmark of Alzheimer’s disease, a progressive neurodegenerative illness primarily caused by the buildup of amyloid plaque, which is brought on by oxidative stress and neuroinflammation. Currently, therapeutic agents are focused on addressing clinical symptoms with associated side effects. This study aims to explore the neuroprotective potential of Ocimum gratissimum, a plant known for its richness in bioactive compounds. By investigating its effects on cognitive health, this study addresses a significant gap in the literature regarding dietary interventions for Alzheimer’s disease. Methods Thirty-six animals were divided into six groups of six mice each: the control group received distilled water intraperitoneally; the scopolamine group received only scopolamine (1 mg/kg i.p.); the three test groups were fed 5%, 10%, and 20% Ocimum gratissimum -supplemented diets while also receiving scopolamine (1 mg/kg i.p.); and the positive control group received Donepezil (5 mg/kg) followed by the injection of scopolamine (1 mg/kg i.p.). Donepezil was administered orally 30 minutes before the scopolamine injection. All treatments were administered daily for 14 consecutive days. Results The supplemented diet groups showed significantly improved spatial memory and navigation compared to the scopolamine-only group. Biochemical analyses revealed that O. gratissimum mitigated scopolamine-induced oxidative stress and neuroinflammation, with marked improvements in antioxidant enzyme levels, reduced lipid peroxidation, and modulation of pro-inflammatory cytokines. Conclusion Dietary intervention using Ocimum gratissimum leaf was able to improve spatial memory and protect against memory impairment, suggesting its potential as a neuroprotective agent against Alzheimer’s disease-like pathology.