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ISOLATION AND IDENTIFICATION OF ANTIMICROBIAL PROTEINS FROM THE LEAVES OF VALERIANA HARDWICKII AND SENNA OBTUSIFOLIA
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Objective : The aim of this study was to screen the antimicrobial activity of trypsin-digested peptides isolated from the protein extracts of Valeriana hardwickii and Senna obtusifolia. Methods: The proteins were extracted from the leaves of V. hardwickii and S. obtusifolia which were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The protein extracts were digested using trypsin which was identified using reverse-phase high-performance liquid chromatography analysis. Further, the antimicrobial efficacy of the digested peptides was investigated.Result: Peptide extracts of V. hardwickii exhibited potent antifungal and antibacterial activity at the maximum concentration of 1.5 mg/ml. Similarly, S. obtusifolia exhibited increased antifungal and antibacterial activity at the concentration of 1.44 mg/ml.Conclusion: The trypsinized extracts of V. hardwickii and S. obtusifolia were plated against Bacillus subtilis which is a promising antibacterial agent.
Title: ISOLATION AND IDENTIFICATION OF ANTIMICROBIAL PROTEINS FROM THE LEAVES OF VALERIANA HARDWICKII AND SENNA OBTUSIFOLIA
Description:
Objective : The aim of this study was to screen the antimicrobial activity of trypsin-digested peptides isolated from the protein extracts of Valeriana hardwickii and Senna obtusifolia.
Methods: The proteins were extracted from the leaves of V.
hardwickii and S.
obtusifolia which were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
The protein extracts were digested using trypsin which was identified using reverse-phase high-performance liquid chromatography analysis.
Further, the antimicrobial efficacy of the digested peptides was investigated.
Result: Peptide extracts of V.
hardwickii exhibited potent antifungal and antibacterial activity at the maximum concentration of 1.
5 mg/ml.
Similarly, S.
obtusifolia exhibited increased antifungal and antibacterial activity at the concentration of 1.
44 mg/ml.
Conclusion: The trypsinized extracts of V.
hardwickii and S.
obtusifolia were plated against Bacillus subtilis which is a promising antibacterial agent.
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