Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Mir-183 functions as an oncogene via decreasing PTEN in breast cancer cells
View through CrossRef
Abstract
Regarding the important role of microRNAs in breast cancer, investigating the molecular mechanisms of miRs and their impacts on breast cancer progression is critical. Thus, the present work aimed to investigate the molecular mechanism of miR-183 in breast cancer. PTEN was validated by dual luciferase assay as a target gene of miR-183. Through qRT-PCR analysis, miR-183 and PTEN mRNA levels in breast cancer cell lines were measured. To determine the impacts of miR-183 on cell viability, the MTT assay was used. Moreover, flowcytometry was applied to analyze the effects of miR-183 on the cell cycle progression. To detect the effects of miR-183 on the migration of BC cell lines, wound healing was used along with a Trans-well migration assay. Western blot was utilized to assess the effect of miR-183 on PTEN protein expression. MiR-183 can exert an oncogenic effect by promoting cell viability, migration, and cell cycle progression. It was revealed that cellular oncogenicity is positively regulated by miR-183 by inhibiting the expression of PTEN. According to the present data, miR-183 may play a vital role in the progression of breast cancer by reducing PTEN expression. It may be also a potential therapeutic target for this disease.
Title: Mir-183 functions as an oncogene via decreasing PTEN in breast cancer cells
Description:
Abstract
Regarding the important role of microRNAs in breast cancer, investigating the molecular mechanisms of miRs and their impacts on breast cancer progression is critical.
Thus, the present work aimed to investigate the molecular mechanism of miR-183 in breast cancer.
PTEN was validated by dual luciferase assay as a target gene of miR-183.
Through qRT-PCR analysis, miR-183 and PTEN mRNA levels in breast cancer cell lines were measured.
To determine the impacts of miR-183 on cell viability, the MTT assay was used.
Moreover, flowcytometry was applied to analyze the effects of miR-183 on the cell cycle progression.
To detect the effects of miR-183 on the migration of BC cell lines, wound healing was used along with a Trans-well migration assay.
Western blot was utilized to assess the effect of miR-183 on PTEN protein expression.
MiR-183 can exert an oncogenic effect by promoting cell viability, migration, and cell cycle progression.
It was revealed that cellular oncogenicity is positively regulated by miR-183 by inhibiting the expression of PTEN.
According to the present data, miR-183 may play a vital role in the progression of breast cancer by reducing PTEN expression.
It may be also a potential therapeutic target for this disease.
Related Results
Abstract B41: Specific blocking of miR-17-5p guide strand in triple negative breast cancer cells, without amplifying passenger strand activity
Abstract B41: Specific blocking of miR-17-5p guide strand in triple negative breast cancer cells, without amplifying passenger strand activity
Abstract
Conventional wisdom holds that only one of the two strands in a microRNA (miRNA) precursor duplex is selected as the active guide strand. The complementary ...
PTEN Is Essential for Normal Cytokine Signaling and Oncogenic Transformation of Pre-B Cells
PTEN Is Essential for Normal Cytokine Signaling and Oncogenic Transformation of Pre-B Cells
Abstract
Background: PTEN is a negative regulator of PI3K-AKT signaling and a potent tumor suppressor throughout all main types of human cancer. Recent work demonstr...
Promoter Methylation and Phosphorylation Status of PTEN in Taiwanese Breast Cancer.
Promoter Methylation and Phosphorylation Status of PTEN in Taiwanese Breast Cancer.
Abstract
Background: The PTEN (phosphatase and tensin homolog deleted on chromosome Ten) gene is a novel candidate of tumor suppressor that plays an important role i...
Coexisting Granulomatous Mastitis and Breast Cancer: A Systematic Review
Coexisting Granulomatous Mastitis and Breast Cancer: A Systematic Review
Abstract
Introduction: Granulomatous mastitis (GM) is a rare inflammatory breast disease that mimics carcinoma. GM can coexist with breast cancer (BC), though the relationship rema...
Abstract 3963: Antisense agents and RNA mimics for miR-17-5p guide strand and miR-17-3p passenger strand differentiate the strength of guide and passenger strand targets in PDCD4 and PTEN mRNA 3′UTRs in MDA-MB-231 triple negative breast cancer cells
Abstract 3963: Antisense agents and RNA mimics for miR-17-5p guide strand and miR-17-3p passenger strand differentiate the strength of guide and passenger strand targets in PDCD4 and PTEN mRNA 3′UTRs in MDA-MB-231 triple negative breast cancer cells
Abstract
Conventional wisdom holds that only one of the two strands in a microRNA (miRNA) precursor duplex is selected as the active guide strand. The complementary ...
MicroRNA-221 and microRNA-222 regulate gastric carcinoma cell proliferation and radioresistance by targeting PTEN
MicroRNA-221 and microRNA-222 regulate gastric carcinoma cell proliferation and radioresistance by targeting PTEN
Abstract
Background
MicroRNAs (miRNAs) can function as either oncogenes or tumor suppressor genes via regulation of cell proliferation and/or apo...
Breast Carcinoma within Fibroadenoma: A Systematic Review
Breast Carcinoma within Fibroadenoma: A Systematic Review
Abstract
Introduction
Fibroadenoma is the most common benign breast lesion; however, it carries a potential risk of malignant transformation. This systematic review provides an ove...
GW24-e2497 Circulating MicroRNAs as Potential Biomarkers of Coagulation Dysfunction in Patients with Vulnerable Coronary Artery Disease
GW24-e2497 Circulating MicroRNAs as Potential Biomarkers of Coagulation Dysfunction in Patients with Vulnerable Coronary Artery Disease
Objectives
The activation of coagulation and fibrinolysis plays a critical role in the incidence of coronary events. MicroRNAs (miRNAs) are small non-coding ribon...