Analytical Synergy: A Review on RP-HPLC Estimation Techniques for Quetiapine and Olanzapine in Bulk and Dosage Forms

A new, simple, specific, sensitive, rapid, accurate and precise RP-HPLC method was developed for the estimation of quetiapine fumarate in bulk and pharmaceutical formulations. Quetiapine fumarate was chromatographed on Microsorb-MV 100-5 C18 (250 x 4.6mm, 5 µm) column using UV detector. The mobile phase consisting acetonitrile and phosphate buffer (pH 3) in the ratio of 50:50 (v/v) at a flow rate of 1.0 ml/min with detection at 292 nm. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness. Olanzapine in pharmaceutical dosage forms can be determined using a reverse phase HPLC technique. Using a 70:30 v/v mixture of methanol and ammonium phosphate buffer as the mobile phase and a flow rate of 1 ml/min, chromatography was performed on an inertsil C18 column. At 220 nm, detection was done. The drug's retention period was 3.447 minutes. In the concentration range of 2 to 10µg/ml of olanzapine, the approach yielded linear results