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Seydoux lab Cas9 preparation test: Tag gtbp?1 with eGFP or mCherry using dpy?10 co?CRISPR v1
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This is a positive control experiment (to test protocol in your hands and/or activity of your home‐made Cas9): tag gtbp‐1 with eGFP or mCherry using dpy‐10 co‐CRISPR.. The protocol is from: Paix A,Folkmann A,Rasoloson D, andSeydoux G (2015) High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR/Cas9 Ribonucleoprotein Complexes.Genetics genetics.115.179382; Early online July 17, 2015.doi:10.1534/genetics.115.179382 Please see the full manuscript for detail.
Title: Seydoux lab Cas9 preparation test: Tag gtbp?1 with eGFP or mCherry using dpy?10 co?CRISPR v1
Description:
This is a positive control experiment (to test protocol in your hands and/or activity of your home‐made Cas9): tag gtbp‐1 with eGFP or mCherry using dpy‐10 co‐CRISPR.
The protocol is from: Paix A,Folkmann A,Rasoloson D, andSeydoux G (2015) High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR/Cas9 Ribonucleoprotein Complexes.
Genetics genetics.
115.
179382; Early online July 17, 2015.
doi:10.
1534/genetics.
115.
179382 Please see the full manuscript for detail.
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