Development and Assessment of Polyherbal Syrup for Hyperlipidemia Management in Animal Model Rabbit

Background: Hyperlipidemia is known as one of major factor causing heart attack and stroke. It is described by an increase in blood cholesterol, low-density Lipoprotein (LDL), and decrease in high-density lipoprotein (HDL) levels. In hypercholesterolemia, elevated cholesterol levels can disrupt neurotransmitter balance, contributing to increased oxidative stress and inflammation in the brain. Certain neurotransmitters, such as serotonin and dopamine, may have antioxidant and anti-inflammatory effects, helping to mitigate these effects. Objective: Formulation and evaluation of herbal syrup for physicochemical, toxicological and efficacy study in hyperlipidemic rabbit model Methodology: In this study polyherbal syrup containing Trigonella foenum-graecum seeds, Allium sativum buds and Aloe vera gel is formulated and further evaluated for its acute oral toxicity and efficacy studies in maleWistar rats and Triton induced hyperlipidemia in rabbits respectively. Results: Polyherbal syrup was developed at laboratory scale further assessed for its physicochemical characteristics like pH, viscosity, density, stability and organoleptic properties. During these studies this formulation was found stable and further used for acute oral toxicity and efficacy studies in albino rats and rabbits. Acute oral toxicity study was conducted in albino wistar female rats as per OECD guidelines 423. Based on clinical findings, it is inferred that the LD50 dose for the herbal syrup exceeds 5,000 mg/kg. For efficacy study, hyperlipidemic rabbit model was prepared by injecting Triton WR-1339 (200 mg/kg i.p.).15 days pretreatment of polyherbal syrup clearly indicates that the cholesterol level was significantly lower in test group (39.2±7.46 mg/dl) and standard group (34.2±3.19 mg/dl) compared with that of control group (119±9.61mg/dl). The triglyceride level was also significantly decreased  in test group (68.2±7.64mg/dl) and standard group (74±19.73mg/dl) groups as compared to control group (185.6±13.9 mg/dl).The low-density Lipoprotein (LDL) level  was also significantly decreased  in test (24.6±6.91mg/dl) and standard groups(43.4±9.36 mg/dl) as compared to control group (127±13.5mg/dl).The high-density lipoprotein (HDL) level was increased in test (27.2±6.30 mg/dl) and standard groups (32.7±7.07 mg/dl) as compared to  control group (17.8±3.96 mg/dl). Conclusion: The results achieved from this study indicate that regular use of this polyherbal syrup may be helpful in managing hyperlipidemia and viable replacement to synthetic medicines.