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Relationship between antimicrobial resistance and virulence factors in uropathogenic Escherichia coli isolates from Ramadi, Iraq: phenotype and genotype identification
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Background: Uropathogenic Escherichia coli (UPEC) is the most predominant pathogen that causes severe urinary tract infections (UTIs). Their therapeutic options are limited due to the rising of antibiotic resistance.
Objective: The aim of the study was to evaluate the level of antibiotic resistance profile, redundancy of virulence genes, and their correlation.
Methods: 41 UPEC isolates were collected from patients diagnosed with UTI, identified by the standard microbiological analysis, and tested for susceptibility to 12 antibiotic agents using the Kirby-Bauer method. The ability of UPEC isolates to produce biofilm, hemolyze and cause clumping of blood was determined. Virulence genes were detected by PCR analysis.
Results: The percentage of UPEC isolates was higher in females (78.1%) than in males (21.9%). UPEC isolates showed a high degree of resistance towards Ceftriaxone (90.2%), Cefepime (90.2%), Ciprofloxacin (82.9%), Levofloxacin (82.9%), and Trimethoprim-Sulfamethoxazole (80.4%). Biofilm formation (87.8%) and hemagglutinin activity (80.4%) were the most predominant virulence markers expressed in UPEC and showed a high degree of correlation with the antibiotic resistance profile. PCR analysis showed that fimH (85.3%) was the most prevalent gene detected in UPEC isolates, followed by aac3-II (80.4%) among the five genes tested, blaTEM, aac3-II, sul2, hlyA, and fimH. The correlation between antibiotic resistant patterns and the presence aac3-II gene was significantly high. The resistance to the sulfonamides’ combined antibiotic was highly correlated with the presence of sulf2 gene.
Conclusion: Antimicrobial resistance was significantly linked to phenotypic and genotypic virulence factors. These results will aid in elucidating the pathogenicity of UTIs and guiding treatment decisions.
Keywords: UPEC resistance profile; virulence factor; Biofilm formation; hemagglutinin; hemolysin.
Title: Relationship between antimicrobial resistance and virulence factors in uropathogenic Escherichia coli isolates from Ramadi, Iraq: phenotype and genotype identification
Description:
Background: Uropathogenic Escherichia coli (UPEC) is the most predominant pathogen that causes severe urinary tract infections (UTIs).
Their therapeutic options are limited due to the rising of antibiotic resistance.
Objective: The aim of the study was to evaluate the level of antibiotic resistance profile, redundancy of virulence genes, and their correlation.
Methods: 41 UPEC isolates were collected from patients diagnosed with UTI, identified by the standard microbiological analysis, and tested for susceptibility to 12 antibiotic agents using the Kirby-Bauer method.
The ability of UPEC isolates to produce biofilm, hemolyze and cause clumping of blood was determined.
Virulence genes were detected by PCR analysis.
Results: The percentage of UPEC isolates was higher in females (78.
1%) than in males (21.
9%).
UPEC isolates showed a high degree of resistance towards Ceftriaxone (90.
2%), Cefepime (90.
2%), Ciprofloxacin (82.
9%), Levofloxacin (82.
9%), and Trimethoprim-Sulfamethoxazole (80.
4%).
Biofilm formation (87.
8%) and hemagglutinin activity (80.
4%) were the most predominant virulence markers expressed in UPEC and showed a high degree of correlation with the antibiotic resistance profile.
PCR analysis showed that fimH (85.
3%) was the most prevalent gene detected in UPEC isolates, followed by aac3-II (80.
4%) among the five genes tested, blaTEM, aac3-II, sul2, hlyA, and fimH.
The correlation between antibiotic resistant patterns and the presence aac3-II gene was significantly high.
The resistance to the sulfonamides’ combined antibiotic was highly correlated with the presence of sulf2 gene.
Conclusion: Antimicrobial resistance was significantly linked to phenotypic and genotypic virulence factors.
These results will aid in elucidating the pathogenicity of UTIs and guiding treatment decisions.
Keywords: UPEC resistance profile; virulence factor; Biofilm formation; hemagglutinin; hemolysin.
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