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Effect of low expression level of acetyl CoA synthetase on secondary metabolite in Monascus.sp
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Acetyl-coenzyme A (acetyl-CoA) is a key metabolite for energy metabolism
and biosynthetic pathways produced by acetyl-CoA synthetase (ACS) gene.
In previous study, ACS gene were speculated to be related to metabolites
production of Monascus sp. In this study, the expression level of ACS
gene was inhibited by RNAi. 10 transformants were picked out to measure
the expression level of ACS gene by RT-qPCR. The ACS gene expression
level of transformant T7 was the lowest, 0.401 times higher than that of
the wild strain. So T7 was selected for the follow-up study. The effects
on the colonial morphology of Monascus had been observed. And the
morphology of mycelia and spores were observed by scanning electron
microscope. The contents of main secondary metabolites of Monascus- MK,
citrinin were determined by HPLC. And the color values of wild-type
strain and T7 transformant have been measured to reflect the content of
pigments by spectrophotometer. The results showed that low expression
level of ACS gene by RNAi could inhibit the growth of Monascus colonies
and could affect the mycelial growth and the type of pigments. The low
expression level of ACS gene could affect the formation and morphology
of M. buliginosus M1 spores. It also could inhibit all the production of
main secondary metabolites of Monascus-MK, citrinin and pigment at the
same time.
Title: Effect of low expression level of acetyl CoA synthetase on secondary metabolite in Monascus.sp
Description:
Acetyl-coenzyme A (acetyl-CoA) is a key metabolite for energy metabolism
and biosynthetic pathways produced by acetyl-CoA synthetase (ACS) gene.
In previous study, ACS gene were speculated to be related to metabolites
production of Monascus sp.
In this study, the expression level of ACS
gene was inhibited by RNAi.
10 transformants were picked out to measure
the expression level of ACS gene by RT-qPCR.
The ACS gene expression
level of transformant T7 was the lowest, 0.
401 times higher than that of
the wild strain.
So T7 was selected for the follow-up study.
The effects
on the colonial morphology of Monascus had been observed.
And the
morphology of mycelia and spores were observed by scanning electron
microscope.
The contents of main secondary metabolites of Monascus- MK,
citrinin were determined by HPLC.
And the color values of wild-type
strain and T7 transformant have been measured to reflect the content of
pigments by spectrophotometer.
The results showed that low expression
level of ACS gene by RNAi could inhibit the growth of Monascus colonies
and could affect the mycelial growth and the type of pigments.
The low
expression level of ACS gene could affect the formation and morphology
of M.
buliginosus M1 spores.
It also could inhibit all the production of
main secondary metabolites of Monascus-MK, citrinin and pigment at the
same time.
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