Molecular Identification of T. mentagrophytes and M. canis Isolated from Children Infected with Tinea Capitis in Basra Governorate by Using AP-PCR Technique

The current study included molecular identification of dermatophytes causing Tinea capitis in children inBasrah governorate, including T. Mentagrophytes&M. canis, using AP-PCR or RAPD –PCR polymerasechain reaction technique. By using EZ-10 Spin Column Fungal Genomic DNA Mini –Preps Kit used toextract DNA from dermatophytes fungi, where DNA was obtained from these two dermatophytes fungi.The DNA bands appeared clearly after the electrophoresis of the DNA extract showed the results of DNAamplification .The efficiency of the primeres used in the diagnosis of these two types of dermatophytes, called: randomprimeres OPAA11, OPAA17, OPU15, OPD18, was obtained by the positive results obtained when amplifiedusing these primeres.Used for both dermatophytes The results obtained by using OPAA17 showed successin DNA amplification - obtained from M.canis and T. Mentagrophytes, with six bands for M.canis. As forT. Mentagrophytes, the number of bands obtained was 13, and the amplification results obtained usingOPAA11 showed the efficiency of this primer.Five bands were obtained when used to amplify the DNA of the dermatophytes. While seven bands belongedto T. Mentagrophytes, The amplification results of OPU15 indicated that there were six bands for M.canis,while only one bande for T. Mentagrophytes showed the results of amplification using OPD18 used indiagnosis of dermatophytes to four bands for M.canis while the number of bands for T. Mentagrophytes hasten bands. This method for identifecation is fast and has a high diagnostic efficiency of dermatophytes, asthe diagnosis based on genetic characteristics is more accurate than resulting on phenotypic characteristics .