Comparative Microbiome Analysis of House Dust Mites, the Most Common Cause of Allergens

Three species of mites, Dermatophagoides farinae, D. pteronyssinus, and Tyrophagus putrescentiae which are most popular in Korean homes, grown under the same condition would show different patterns of microbiome, and induce different immune reactions. Three species of mites (D. farinae, D. pteronyssinus, and T. putrescentiae), known to cause allergies, are cultured in s same condition (autoclaved media, 80%RH, 25_) and, analyzed for microbiome of each species. Using the next generation sequencing, the difference of microbiome between the dust mite species, and the level of endotoxin were investigated. The extracts of three species of mites were treated to human bronchial epithelial cells (BEAS 2B cells) to measure the expression level of proinflammatory cytokines. Three species of mites, D. farinae, D. pteronyssinus, and T. putrescentiae, grown under the same condition showed significantly different patterns of microbiome. In D. pteronyssinus grown on the autoclaved medium, only a few bacteria were seen in contrast to the other two species. The concentration of endotoxin derived from the extract of mites was proportional to the distribution of the microbiome. The expressed cytokine levels from BEAS 2B cell were comparable among three species of mites. The microbiome pattern of laboratory cultured dust mites differ not on the culture conditions but on the species. The extent to induce Th2 responses in vitro which may closely be related in allergic responses in humans also showed difference.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.