Free radical scavenging compounds from Ochna integerrima

By repetitive chromatography, a total of 19 pure compounds were isolated from the leaves, stem wood, stem bark, root wood and root bark of O. integerrima. The structures of these compounds were determined by spectroscopic methods (UV, IR, MS and NMR). They included two new compounds, 6‴-hydroxylophirone B and 6‴-hydroxylophirone B 4‴-O-β-glucoside and seventeen known compounds, namely lophirone C, ochnaflavone, calodenone, 5-hydroxy-4′-methoxy-6, 7-methylenedioxy isoflavone, lophirone A, 7″-O-methyl ochnaflavone, squarrosin, 5,3′,4′-trimethoxy-6,7-methylenedioxy isoflavone, 3,3′,4′,5,7-pentahydroxy-6-prenylflavanone, 3-(2,4-dihydroxybenzoyl)-4,6-dihydroxy-2-(4-hydroxyphenyl)-1-benzofuran-7-yl 2-(4-hydroxyphenyl) ethenyl ketone, 3-(2,4-dihydroxybenzoyl)-2,3-dihydro-4,6-dihydroxy-2-(4-hydroxyphenyl)-1-benzofuran-7-yl 2-(4-hydroxyphenyl) ethenyl ketone, 5,4′-dimethoxy-6, 7-methylenedioxy isoflavone, gerontoisoflavone A, 4′,7-dihydroxy 5-methoxy isoflavone, trans tetracocyl ferulate, 2,7,4′-trihydroxy isoflavone and protocatechuic acid. 3-(2,4-Dihydroxybenzoyl)-4,6-dihydroxy-2-(4-hydroxyphenyl)-1-benzofuran-7-yl 2-(4-hydroxyphenyl) ethenyl ketone, 4′,7-dihydroxy 5-methoxy isoflavone and 2,7,4′-trihydroxy isoflavone exhibited moderated DPPH radical scavenging activity.