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Investigation of molecular variability in some Aegilops species using Start Codon Targeted Polymorphism (SCoT) and CAAT-Box Derived Polymorphism (CBDP) markers
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Among wild relatives of wheat, Aegilops species are ideal genetic resources
for the discovery of new characteristics such as resistance to environmental
stresses and even grain quality for wheat improvement. Hence, knowledge of
the population structure and genetic diversity of this germplasm is very
important for their conservation and further utilization. In the present
study, 80 accessions of the Aegilops including Ae. tauschii, Ae. cylindrica
and Ae. triuncialis were investigated for genetic diversity using SCoT and
CBDP markers. Eight SCOT and twelve CBDP primers amplified a total of 84 and
94 fragments with a mean of 10.50 and 7.83 fragments per primer,
respectively. Resolving power (Rp) for SCoT and CBDP primers varied between
6.04 and 11.65, and 13.08 and 28.02, with the polymorphic information
content (PIC) from 0.40 to 0.49 and 0.35 to 0.48, respectively. The results
of analysis of molecular variance (AMOVA) indicated that the highest
proportion of genetic variance referred to between species. SCoT primers
indicated high values for all informativeness parameter (except resolving
power) than CBDP primers across all tested accessions. However, CBDP primers
indicated higher values of the genetic parameters than using SCoT primers.
As a result, the maximum values for genetic parameters such as number of
effective alleles (Ne), Nei?s gene diversity (H) and Shannon?s information
index (I) were detected in Ae. cylindrica and Ae. triuncialis using SCoT and
CBDP markers, respectively. Cluster analysis based on those molecular system
grouped all accessions into three main clusters. The grouping pattern
observed by CBDP primers indicated more clear phylogenetic relationship
among some Aegilops species, so that PCoA?s results confirmed the grouping
pattern. In conclusion, it was observed that SCoT and CBDP displayed good
efficiency in depicting polymorphism among the tested accessions, however,
CBDP markers provided a clear grouping pattern of evaluated accessions.
Hence, the use of CBDP markers in determining population structure and
estimating genetic diversity in other plant species is recommended.
National Library of Serbia
Title: Investigation of molecular variability in some Aegilops species using Start Codon Targeted Polymorphism (SCoT) and CAAT-Box Derived Polymorphism (CBDP) markers
Description:
Among wild relatives of wheat, Aegilops species are ideal genetic resources
for the discovery of new characteristics such as resistance to environmental
stresses and even grain quality for wheat improvement.
Hence, knowledge of
the population structure and genetic diversity of this germplasm is very
important for their conservation and further utilization.
In the present
study, 80 accessions of the Aegilops including Ae.
tauschii, Ae.
cylindrica
and Ae.
triuncialis were investigated for genetic diversity using SCoT and
CBDP markers.
Eight SCOT and twelve CBDP primers amplified a total of 84 and
94 fragments with a mean of 10.
50 and 7.
83 fragments per primer,
respectively.
Resolving power (Rp) for SCoT and CBDP primers varied between
6.
04 and 11.
65, and 13.
08 and 28.
02, with the polymorphic information
content (PIC) from 0.
40 to 0.
49 and 0.
35 to 0.
48, respectively.
The results
of analysis of molecular variance (AMOVA) indicated that the highest
proportion of genetic variance referred to between species.
SCoT primers
indicated high values for all informativeness parameter (except resolving
power) than CBDP primers across all tested accessions.
However, CBDP primers
indicated higher values of the genetic parameters than using SCoT primers.
As a result, the maximum values for genetic parameters such as number of
effective alleles (Ne), Nei?s gene diversity (H) and Shannon?s information
index (I) were detected in Ae.
cylindrica and Ae.
triuncialis using SCoT and
CBDP markers, respectively.
Cluster analysis based on those molecular system
grouped all accessions into three main clusters.
The grouping pattern
observed by CBDP primers indicated more clear phylogenetic relationship
among some Aegilops species, so that PCoA?s results confirmed the grouping
pattern.
In conclusion, it was observed that SCoT and CBDP displayed good
efficiency in depicting polymorphism among the tested accessions, however,
CBDP markers provided a clear grouping pattern of evaluated accessions.
Hence, the use of CBDP markers in determining population structure and
estimating genetic diversity in other plant species is recommended.
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