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Analysis of (Allium cepa × A. fistulosum) × A. cepa Fourth-generation Backcross Populations for A. fistulosum Introgression
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(
Allium cepa
×
A. fistulosum
) ×
A. cepa
breeding lines have been established to the fourth generation. The aim is to develop an
A. cepa
-like bulbing onion carrying
A. fistulosum
genes. Seven populations were characterized for morphological traits and three isozyme markers. Each bulb from the populations was characterized for maturity, soluble solids content, bulb shape, and bulb color. All the populations produced
A. cepa
-like bulbs. Significant variation was observed within each population for each morphological trait. All the bulbs were screened for the presence of
A. cepa
and
A. fistulosum
alleles of alcohol dehydrogenase (Adh-1), esterase (Est), and phosphoglucoisomerase (Pgi-1).
Allium cepa
Adh-1, Est, and Pgi-1 alleles were observed in all the populations. One population, 951026-8, contained plants heterozygous for
A. cepa
and
A. fistulosum
Pgi-1 alleles. Recovery of these fourth generation Allium backcross plants demonstrates introgression of the
A. fistulosum
genome into an
A. cepa
-like bulbing onion.
Title: Analysis of (Allium cepa × A. fistulosum) × A. cepa Fourth-generation Backcross Populations for A. fistulosum Introgression
Description:
(
Allium cepa
×
A.
fistulosum
) ×
A.
cepa
breeding lines have been established to the fourth generation.
The aim is to develop an
A.
cepa
-like bulbing onion carrying
A.
fistulosum
genes.
Seven populations were characterized for morphological traits and three isozyme markers.
Each bulb from the populations was characterized for maturity, soluble solids content, bulb shape, and bulb color.
All the populations produced
A.
cepa
-like bulbs.
Significant variation was observed within each population for each morphological trait.
All the bulbs were screened for the presence of
A.
cepa
and
A.
fistulosum
alleles of alcohol dehydrogenase (Adh-1), esterase (Est), and phosphoglucoisomerase (Pgi-1).
Allium cepa
Adh-1, Est, and Pgi-1 alleles were observed in all the populations.
One population, 951026-8, contained plants heterozygous for
A.
cepa
and
A.
fistulosum
Pgi-1 alleles.
Recovery of these fourth generation Allium backcross plants demonstrates introgression of the
A.
fistulosum
genome into an
A.
cepa
-like bulbing onion.
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