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Species of Fusarium and Neocosmospora associated with citrus branch diseases in China

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Fig. S1. Phylogenetic tree generated by Bayesian inference analyses based on the individual CaM, rpb1, rpb2 and tef1 (A–D) for species in Fusarium fujikuroi species complex (FFSC). Bayesian posterior probability (PP ≥ 0.9) and IQ-TREE bootstrap support values (ML ≥ 70 %) presented above branches as follows: PP/ ML. PP < 0.9 and ML < 70 % of branches marked with *. Newly generated sequences are in blue. Ex-type and ex-epitype strains are indicated with T and ET, respectively. The tree is rooted to F. nirenbergiae (CBS 840.88). <br />Fig. S2. Phylogenetic tree generated by Bayesian inference analyses based on the individual CaM, rpb2 and tef1 (A–C) for species in Fusarium incarnatum-equiseti species complex (FIESC). Bayesian posterior probability (PP ≥ 0.9) and IQ-TREE bootstrap support values (ML ≥ 70 %) presented above branches as follows: PP/ML. PP < 0.9 and ML < 70 % of branches marked with *. Newly generated sequences are in blue. Ex-type, exepitype and ex-neotype strains are indicated with T, ET and NT respectively. The tree is rooted to F. nodosum (CBS 201.63). <br />Fig. S3. Phylogenetic tree generated by Bayesian inference analyses based on the individual rpb2, tef1 and tub2 (A–C) for species in Fusarium lateritium species complex (FLSC). Bayesian posterior probability (PP ≥ 0.9) and IQ-TREE bootstrap support values (ML ≥ 70 %) presented above branches as follows: PP/ML. PP < 0.9 and ML < 70 % of branches marked with *. Newly generated sequences are in blue. Ex-type, ex-epitype and ex-neotype strains are indicated with T, ET and NT respectively. The tree is rooted to F. acuminatum (LC5227). <br />Fig. S4. Phylogenetic tree generated by Bayesian inference analyses based on the individual CaM, rpb1, rpb2 and tef1 (A–D) for species in Fusarium oxysporum species complex (FOSC). Bayesian posterior probability (PP ≥ 0.9) and IQ-TREE bootstrap support values (ML ≥ 70 %) presented above branches as follows: PP/ML. PP < 0.9 and ML < 70 % of branches marked with *. Newly generated sequences are in blue. Ex-type and exepitype strains are indicated with T and ET, respectively. The tree is rooted to F. campestre (KG158). <br />Fig. S5. Phylogenetic tree generated by Bayesian inference analyses based on the individual rpb1, rpb2 and tef1 (A–C) for species in Fusarium sambucinum species complex (FSAMSC). Bayesian posterior probability (PP ≥ 0.9) and IQ-TREE bootstrap support values (ML ≥ 70 %) presented above branches as follows: PP/ ML. PP < 0.9 and ML < 70 % of branches marked with *. Newly generated sequences are in blue and ex-type and ex-epitype strains are indicated with T and ET, respectively. The tree is rooted to F. kotabaruense (Indo172). <br />Fig. S6. Phylogenetic tree generated by Bayesian inference analyses based on the individual rpb1, rpb2 and tef1 (A–C) for species in Fusarium tricinctum species complex (FTSC). Bayesian posterior probability (PP ≥ 0.9) and IQ-TREE bootstrap support values (ML ≥ 70 %) presented above branches as follows: PP/ ML. PP < 0.9 and ML < 70 % of branches marked with *. Newly generated sequences are in blue. Ex-type, ex-epitype and exneotype strains are indicated with T, ET and NT respectively. The tree is rooted to F. foetens (CBS 110286). <br />Fig. S7. Phylogenetic tree generated by Bayesian inference analyses based on the individual ITS, LSU, rpb2 and tef1 (A–D) for species in Neocosmospora. Bayesian posterior probability (PP ≥ 0.9) and IQ-TREE bootstrap support values (ML ≥ 70 %) presented above branches as follows: PP/ML. PP < 0.9 and ML < 70 % of branches marked with *. Newly generated sequences are in blue. Ex-type and ex-epitype strains are indicated with T and ET, respectively. The tree is rooted to Setofusarium setosum (CBS 635.92). <br />Table S1. Details of all fusarioid isolates obtained in this study. <br />Table S2. Fusarioid species isolated from citrus with branch canker in different orchards across different provinces.
Westerdijk Fungal Biodiversity Institute
Title: Species of Fusarium and Neocosmospora associated with citrus branch diseases in China
Description:
Fig.
S1.
Phylogenetic tree generated by Bayesian inference analyses based on the individual CaM, rpb1, rpb2 and tef1 (A–D) for species in Fusarium fujikuroi species complex (FFSC).
Bayesian posterior probability (PP ≥ 0.
9) and IQ-TREE bootstrap support values (ML ≥ 70 %) presented above branches as follows: PP/ ML.
PP < 0.
9 and ML < 70 % of branches marked with *.
Newly generated sequences are in blue.
Ex-type and ex-epitype strains are indicated with T and ET, respectively.
The tree is rooted to F.
nirenbergiae (CBS 840.
88).
<br />Fig.
S2.
Phylogenetic tree generated by Bayesian inference analyses based on the individual CaM, rpb2 and tef1 (A–C) for species in Fusarium incarnatum-equiseti species complex (FIESC).
Bayesian posterior probability (PP ≥ 0.
9) and IQ-TREE bootstrap support values (ML ≥ 70 %) presented above branches as follows: PP/ML.
PP < 0.
9 and ML < 70 % of branches marked with *.
Newly generated sequences are in blue.
Ex-type, exepitype and ex-neotype strains are indicated with T, ET and NT respectively.
The tree is rooted to F.
nodosum (CBS 201.
63).
<br />Fig.
S3.
Phylogenetic tree generated by Bayesian inference analyses based on the individual rpb2, tef1 and tub2 (A–C) for species in Fusarium lateritium species complex (FLSC).
Bayesian posterior probability (PP ≥ 0.
9) and IQ-TREE bootstrap support values (ML ≥ 70 %) presented above branches as follows: PP/ML.
PP < 0.
9 and ML < 70 % of branches marked with *.
Newly generated sequences are in blue.
Ex-type, ex-epitype and ex-neotype strains are indicated with T, ET and NT respectively.
The tree is rooted to F.
acuminatum (LC5227).
<br />Fig.
S4.
Phylogenetic tree generated by Bayesian inference analyses based on the individual CaM, rpb1, rpb2 and tef1 (A–D) for species in Fusarium oxysporum species complex (FOSC).
Bayesian posterior probability (PP ≥ 0.
9) and IQ-TREE bootstrap support values (ML ≥ 70 %) presented above branches as follows: PP/ML.
PP < 0.
9 and ML < 70 % of branches marked with *.
Newly generated sequences are in blue.
Ex-type and exepitype strains are indicated with T and ET, respectively.
The tree is rooted to F.
campestre (KG158).
<br />Fig.
S5.
Phylogenetic tree generated by Bayesian inference analyses based on the individual rpb1, rpb2 and tef1 (A–C) for species in Fusarium sambucinum species complex (FSAMSC).
Bayesian posterior probability (PP ≥ 0.
9) and IQ-TREE bootstrap support values (ML ≥ 70 %) presented above branches as follows: PP/ ML.
PP < 0.
9 and ML < 70 % of branches marked with *.
Newly generated sequences are in blue and ex-type and ex-epitype strains are indicated with T and ET, respectively.
The tree is rooted to F.
kotabaruense (Indo172).
<br />Fig.
S6.
Phylogenetic tree generated by Bayesian inference analyses based on the individual rpb1, rpb2 and tef1 (A–C) for species in Fusarium tricinctum species complex (FTSC).
Bayesian posterior probability (PP ≥ 0.
9) and IQ-TREE bootstrap support values (ML ≥ 70 %) presented above branches as follows: PP/ ML.
PP < 0.
9 and ML < 70 % of branches marked with *.
Newly generated sequences are in blue.
Ex-type, ex-epitype and exneotype strains are indicated with T, ET and NT respectively.
The tree is rooted to F.
foetens (CBS 110286).
<br />Fig.
S7.
Phylogenetic tree generated by Bayesian inference analyses based on the individual ITS, LSU, rpb2 and tef1 (A–D) for species in Neocosmospora.
Bayesian posterior probability (PP ≥ 0.
9) and IQ-TREE bootstrap support values (ML ≥ 70 %) presented above branches as follows: PP/ML.
PP < 0.
9 and ML < 70 % of branches marked with *.
Newly generated sequences are in blue.
Ex-type and ex-epitype strains are indicated with T and ET, respectively.
The tree is rooted to Setofusarium setosum (CBS 635.
92).
<br />Table S1.
Details of all fusarioid isolates obtained in this study.
<br />Table S2.
Fusarioid species isolated from citrus with branch canker in different orchards across different provinces.

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