Altered transcriptome responses in the lungs of preweaned calves supplemented with a yeast fermentate during a viral-bacterial coinfection

Abstract Bovine respiratory disease is a complex syndrome which contributes to severe and often fatal pneumonia in beef and dairy cattle. Saccharomyces cerevisiae fermentation products (SCFP) are feed ingredients that have been previously shown to alter the immune response and outcome of a respiratory viral infection in calves. The objective of this study was to determine the effect of SCFP supplementation on the lung transcriptome in calves responding to a co-infection with bovine respiratory syncytial virus (BRSV) and Pasteurella multocida (PM). Twenty-eight, 1–2 day old calves were assigned to two groups: 1) control diet; or 2) SCFP treated diet. Calves were infected with BRSV on day 21, followed 6 days later by intratracheal inoculation with PM. Calves were euthanized on day 10 post-viral infection. There were no differences in viral or bacterial burden between treatment groups, but SCFP treated calves tended to have less severe lung pathology. Transcriptome analysis of lung tissue and bronchoalveolar lavage samples revealed over-represented genes related to membrane integrity, pathogen binding, and complement activation which presumably contributes to the host’s resiliency and response to the coinfection. Results from this study suggest that supplementing preweaned calves with SCFP may modulate the innate and adaptive immune response in mucosal sites. These findings will contribute to a better understanding of the underlying host response to bovine respiratory disease. Supported by funding from Diamond V Mills, Inc., Cedar Rapids, IA