Inhibitory activity and degradation of curcumin as Anti-Biofilm Polymicrobial on Catheters

Related biofilms in urinary tract infections and cause deaths per 7500 people. Biofilms are a serious problem and are resolved with antibiotic therapy. Curcumin is a pure composition of the turmeric plant (Curcuma longa Linn.) Which has antimicrobial activity, but the activity of polymicrobial antibiofilm on the catheter has never been launched. The discovery of new antibiofilm candidates for polymicrobial biofilms on catheters is a challenge that must be overcome in preventing infections related to biofilms.  This study aims to determine the effectiveness of curcumin in inhibiting and degrading polymicrobial catheters. Biofilm inhibition testing and biofilm degradation testing were determined using the microtiter broth method. The effectiveness of curcumin on biofilms was analyzed by calculating the minimum biofilm inhibitory concentration (MBIC50) and the minimum value of biofilm eradication concentration (MBEC50). The mechanism of action of curcumin against polymicrobial biofilms on the catheter was tested using scanning electron microscopy (SEM). Curcumin 1% gives 60% inhibitory activity to the formation of polymicrobial biofilms on the catheter in the middle phase by 65.05 ± 0.01 and the maturation phase by 61.23 ± 0.01, and this is better than the control of the drug nystatin in the middle phase and maturation by 57.58 ± 0.01 and 56.31 ± 0.01 and are almost equivalent to the control drug chloramphenicol. The results also provide evidence of the activity of curcumin can degrade 50%. Polymicrobial biofilm on the catheter was 51.49 ± 0.01 and damaged the extracellular polymeric matrix (EPS) polymicrobial biofilm on the catheter. Therefore, curcumin is very potential to be developed as a candidate for new antibiofilm drugs against polymicrobial catheters