Longitudinal Evaluation of Dravya and Guna Samanya Principles: A Drosophila melanogaster Model of Tissue Accretion Using Aja Mamsa (Capra hircus meat) and Masha (Vigna mungo).

The Samanya Vishesha Siddhanta is a basic therapeutic principle of Ayurveda wherein similarityleads to accumulation of tissues and dissimilarity leads to depletion. The present study is anattempt to evaluate two traditional methods of treatment for tissue wasting (Dhatu Kshaya) in alongitudinal way using Dravya Samanya (substance homology) with AjaMamsa (goat meat) andGuna Samanya (quality homology) with Masha (black gram).The study used an in vivo model of Drosophila melanogaster (N=400 wild type flies). The flieswere randomised into four groups: Full diet control (C1), Nutrient restricted half diet control(C2), Masha Vesavara Test group (T1) and AjaMamsa Vesavara Test group (T2). Test diets wereformulated with 10% w/v of the respective extracts. Morphometric data (total body weight, axislength, abdominal width and Body Mass Index (BMI)) were collected over a 30-day period, andanalysed by Two-Way Repeated Measures ANOVA and Tukey's HSD post-hoc test. Thenutrient-restricted cohort (C2) exhibited a steady decline in body mass by 5.88% at Day 30. Boththe AjaMamsa and Masha treatments reversed this wastage trend and resulted in significant gainsin mass. However, the AjaMamsa cohort (T2) showed a significantly better gain of 24.75% massgain as against the 19.00% gain in the Masha cohort (T1) (p<0.01) .The AjaMamsa group also showed large increases in abdominal width. The trends in BMI weresimilar for all the groups for the 30 days thus eliminating isolated and unhealthy deposition oflipids and confirming the proportional and healthy structural tissue (Mamsa Vardhana). Theempirical data support the therapeutic superiority of Dravya Samanya (AjaMamsa) over GunaSamanya (Masha) in building and restoring the tissue mass. These findings provide support foran evidence-based clinical hierarchy for treatment of structural wasting, with direct homologoussubstances being the primary intervention.